Browning of the subcutaneous adipocytes in diet-induced obese mouse submitted to intermittent fasting
| Autor: | Fernanda Ornellas, Marcia Barbosa Aguila, Carlos Alberto Mandarim-de-Lacerda, Thatiany de Souza Marinho |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Adipocytes White Subcutaneous Fat Adipose tissue 030209 endocrinology & metabolism White adipose tissue Diet High-Fat Biochemistry 03 medical and health sciences Mice 0302 clinical medicine Endocrinology Internal medicine Intermittent fasting medicine Animals Obesity Molecular Biology Adiponectin business.industry Leptin Thermogenesis Fasting Thermogenin Mice Inbred C57BL 030104 developmental biology Adipocytes Brown Cell Transdifferentiation business Energy Intake Diet-induced obese |
| Zdroj: | Molecular and cellular endocrinology. 513 |
| ISSN: | 1872-8057 |
| Popis: | Purpose We studied subcutaneous white adipose tissue (sWAT) of obese mice submitted to intermittent fasting (IF). Methods Twelve-week-old C57BL/6 male mice received the diets Control (C) or high-fat (HF) for eight weeks (n = 20/each). Then, part of each group performed IF (24 h feeding/24 h fasting) for four weeks: C, C-IF, HF, and HF-IF (n = 10/each). Results Food intake did not show a difference in feeding and fasting days, but HF groups had a high energy intake. IF led to multilocular adipocytes in sWAT (browning), and improved respiratory quotient on the fed day. IF decreased gene expression of Leptin, but increased Adiponectin, β3ar (beta3 adrenoreceptor), and Ucp1 (uncoupling protein). IF enhanced immunostaining of Caspase 3, Pcna (proliferating cell nuclear antigen), and UCP1 in sWAT. IF attenuated pro-inflammatory markers and pro-apoptotic markers in sWAT. Conclusions IF in obese mice led to browning in sWAT adipocytes, enhanced thermogenesis, an improved adipose tissue pro-inflammatory profile. |
| Databáze: | OpenAIRE |
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