Evidence for absence of the MPB64 gene in some substrains of Mycobacterium bovis BCG
| Autor: | Morten Harboe, S. Nagai, J. C. Ulstrup, K. Melby, Huayi Li, T. Ø. Jonassen |
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| Rok vydání: | 1993 |
| Předmět: |
Tuberculosis
Immunology Molecular Sequence Data Biology Microbiology law.invention Mycobacterium tuberculosis Gene product Bacterial Proteins Species Specificity law RNA Ribosomal 16S medicine Gene Polymerase chain reaction Mycobacterium bovis Antigens Bacterial Base Sequence medicine.disease biology.organism_classification Infectious Diseases Secretory protein Oligodeoxyribonucleotides Genes Bacterial Parasitology Bacteria Research Article |
| Zdroj: | Infection and immunity. 61(5) |
| ISSN: | 0019-9567 |
| Popis: | Substrains of Mycobacterium bovis BCG have been divided in two major groups, high producers and low producers of the secreted proteins MPB64 and MPB70. Of these, Mycobacterium tuberculosis secretes only the analog MPT64 during growth on Sauton medium. It has been confirmed that high-producer and low-producer substrains of BCG as well as M. tuberculosis contain the gene for the MPB/MPT70 protein. By contrast, polymerase chain reaction and hybridization experiments are reported here which indicate that the MPB64 gene is absent in the BCG substrains Copenhagen, Pasteur, Glaxo, and Tice, in which previous methods did not permit distinction between secretion of small amounts or absence of the protein in culture fluids. |
| Databáze: | OpenAIRE |
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