A porphyrin‐based fluorescence method for zinc determination in commercial propolis extracts without sample pretreatment
| Autor: | Marcelo Fabian Pistonesi, Júlio S. Rebouças, Maria Susana Di Nezio, Victor Hugo Araújo Pinto, Wallace D. Fragoso, Gastón Darío Pierini, Clarissa G.C. Maia, María Eugenia Centurión |
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| Rok vydání: | 2017 |
| Předmět: |
Porphyrins
Calibration curve Biophysics chemistry.chemical_element Zinc 010402 general chemistry 01 natural sciences Propolis law.invention chemistry.chemical_compound law Fluorescent Dyes Reproducibility Chromatography Spectrophotometry Atomic 010401 analytical chemistry Reproducibility of Results Repeatability Fluorescence Porphyrin 0104 chemical sciences Spectrometry Fluorescence Mesoporphyrins chemistry Chemistry (miscellaneous) Calibration Spectrophotometry Ultraviolet Atomic absorption spectroscopy Brazil |
| Zdroj: | Luminescence. 32:1227-1232 |
| ISSN: | 1522-7243 1522-7235 |
| Popis: | The quantification of zinc in over-the–counter drugs as commercial propolis extracts by molecular fluorescence technique using meso-tetrakis(4-carboxyphenyl)porphyrin (H2TCPP4) was developed for the first time. The calibration curve is linear from 6.60 to 100 nmol L−1 of Zn2+. The detection and quantification limits were 6.22 nmol L−1 and 19.0 nmol L−1, respectively. The reproducibility and repeatability calculated as the percentage variation of slopes of seven calibration curves were 6.75% and 4.61%, respectively. Commercial propolis extract samples from four Brazilian states were analyzed and the results (0.329–0.797 mg/100 mL) obtained with this method are in good agreement with that obtained with the Atomic Absorption Spectroscopy (AAS) technique. The method is simple, fast, of low cost and allows the analysis of the samples without pretreatment. Moreover the major advantage is that Zn-porphyrin complex presents fluorescent characteristic promoting the selectivity and sensitivity of the method. |
| Databáze: | OpenAIRE |
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