Role of p16(INK4a) in the inhibition of DNA synthesis stimulated by HGF or EGF in primary cultured rat hepatocytes
| Autor: | Taiichiro Seki, Toyohiko Ariga, Shingo Niimi, Mizuho Harashima |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
DNA Replication
Messenger RNA Small interfering RNA DNA synthesis Epidermal Growth Factor Chemistry Hepatocyte Growth Factor RNA General Medicine Transfection Molecular biology General Biochemistry Genetics and Molecular Biology Rats Gene Expression Regulation Epidermal growth factor RNA interference embryonic structures medicine Hepatocytes Animals Hepatocyte growth factor RNA Interference RNA Messenger Cyclin-Dependent Kinase Inhibitor p16 medicine.drug |
| Zdroj: | Biomedical research (Tokyo, Japan). 34(5) |
| ISSN: | 1880-313X |
| Popis: | In the present study, we investigated the role of p16(INK4a) in the inhibition of DNA synthesis stimulated by hepatocyte growth factor (HGF) or epidermal growth factor (EGF) using RNA interference in primary cultured rat hepatocytes. The transfection of small interfering RNAs targeting p16(INK4a) reduced the corresponding mRNA and protein expression by more than approximately 90% and 50%, respectively, at 24 h after transfection. In the cells transfected with p16(INK4a) small interfering RNA, control, HGF, and EGF-stimulated DNA synthesis as assessed by (3)H-thymidine incorporation increased by approximately 1.5-fold, 1.6-fold, and 1.7-fold, respectively, compared with that in the control small interfering RNA-transfected cells. These findings indicate that p16(INK4a) plays a significant role in the inhibition of DNA synthesis. |
| Databáze: | OpenAIRE |
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