Factors Influencing Microbiological Biodiversity of Human Foot Skin
| Autor: | Anna Otlewska, Justyna Szulc, Anita Jachowicz, Aleksandra Steglińska, Beata Gutarowska, Justyna Adamiak, Katarzyna Pielech-Przybylska |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Veterinary medicine Adolescent Firmicutes Health Toxicology and Mutagenesis Microorganism lcsh:Medicine Article Actinobacteria Young Adult 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Culture Techniques RNA Ribosomal 16S Proteobacteria Humans Child microorganisms foot skin Skin biodiversity 030304 developmental biology 0303 health sciences Bacteria biology Ascomycota Foot Microbiota lcsh:R Fungi Public Health Environmental and Occupational Health High-Throughput Nucleotide Sequencing Infant high-throughput sequencing Middle Aged biology.organism_classification Antimicrobial Child Preschool Female Foot (unit) |
| Zdroj: | International Journal of Environmental Research and Public Health, Vol 16, Iss 18, p 3503 (2019) International Journal of Environmental Research and Public Health Volume 16 Issue 18 |
| ISSN: | 1660-4601 |
| Popis: | The aim of the study was to analyze the microbiological biodiversity of human foot skin with respect to factors such as age, gender, frequency of foot washing and physical activity, and to select indicator species to be considered when designing textile materials with antimicrobial properties used for sock and insole production. The experiment was carried out on a group of 40 people. The number of microorganisms was determined using culture-dependent methods. Biodiversity was determined using culture followed by genetic identification based on 16S rRNA gene sequencing (bacteria), ITS region (fungi), or using Illumina next-generation sequencing (in a group of eight selected individuals). The total bacterial number on women&rsquo s feet was on average 1.0 × 106 CFU/cm2, and was not statistically significantly different than that of men&rsquo s feet (mean 1.2 × 105 CFU/cm2). The number of bacteria, in most cases, decreased with age and with increased frequency of physical activity. The number of bacteria increased with diminishing feet-washing frequency however, statistically significant differences were found between groups. The number of fungi was not significantly different amongst groups. Bacteria belonging to the phyla Firmicutes, Proteobacteria and Actinobacteria constituted the main microorganisms of the foot skin. Ascomycota and Basidiomycota predominated amongst the fungi. The presence of specific species varied in groups depending on the factors mentioned above. Two of the species identified were classified as pathogens (Neisseria flavescens and Aspergillus fumigatus). These findings suggest that it is necessary to extend the list of microorganisms tested on textiles with respect to hygienic properties. |
| Databáze: | OpenAIRE |
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