Streptozotocin-induced leukocyte DNA damage in rats
| Autor: | Gustavo Tadeu Volpato, Yuri Karen Sinzato, Débora Cristina Damasceno, Rafael Bottaro Gelaleti, Emilio Herrera, Marilza Vieira Cunha Rudge |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Federal University of Mato Grosso (UFMT), Universidad San Pablo-CEU |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Blood Glucose
Time Factors endocrine system diseases DNA damage Health Toxicology and Mutagenesis 010501 environmental sciences Pharmacology Toxicology medicine.disease_cause 01 natural sciences streptozotocin Streptozocin 03 medical and health sciences 0302 clinical medicine comet assay Leukocytes medicine Animals Rats Wistar 0105 earth and related environmental sciences Glycemic Chemical Health and Safety Mutagenicity Tests Chemistry Public Health Environmental and Occupational Health nutritional and metabolic diseases General Medicine Streptozotocin Hypoglycemia Peripheral blood Rats Comet assay rats Hyperglycemia Female Comet Assay hyperglycemia Genotoxicity 030217 neurology & neurosurgery DNA Damage medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T17:22:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Although several studies using peripheral blood samples suggest that DNA damage is caused by streptozotocin (STZ) per se, our hypothesis is that DNA damage is caused by STZ-induced glycemic changes. Thus, we aimed at evaluating DNA damage levels in peripheral blood samples from rats at different time points within the first 24 h after a single intravenous dose of STZ. Female Wistar rats (control, n = 8; STZ, n = 7) were administered a single STZ intravenous injection (40 mg/kg body weight). Blood samples were collected from the tail vein for genotoxicity analysis by comet assay and glycemia assessment before STZ administration (time point zero) and at 2, 4, 6, 8, 12, and 24 h afterward. At 2 h, there was initial hyperglycemia associated with STZ-induced glycogenolysis that caused an increase in leukocyte DNA damage levels. At 4 h, glycemic and DNA damage levels were normalized. However, at 6 and 8 h, we observed hypoglycemia concomitant with increased DNA damage levels. From 10 h onward up to 24 h, DNA damage persisted and hyperglycemia appeared. Thus, DNA damage increased soon after both hypoglycemia and hyperglycemia, which were not directly induced by STZ owing to its known short life. In conclusion, increased peripheral blood DNA damage levels within 24 h after STZ administration in rats are associated with abnormal glycemic levels and their complications rather than with STZ per se. Laboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School UNESP_Univ Estadual Paulista Laboratory of System Physiology and Reproductive Toxicology Institute of Biological and Health Sciences Federal University of Mato Grosso (UFMT) Faculties of Pharmacy and Medicine Universidad San Pablo-CEU Laboratory of Experimental Research on Gynecology and Obstetrics Botucatu Medical School UNESP_Univ Estadual Paulista |
| Databáze: | OpenAIRE |
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