Controlling Macrophage Polarization to Modulate Inflammatory Cues Using Immune-Switch Nanoparticles
| Autor: | Ana F. Almeida, Margarida S. Miranda, Adriana Vinhas, Ana I. Gonçalves, Manuela E. Gomes, Márcia T. Rodrigues |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Inflammation
Macrophages Organic Chemistry General Medicine Macrophage Activation Catalysis Computer Science Applications Inorganic Chemistry Humans Nanoparticles Physical and Theoretical Chemistry Inflammation Mediators Molecular Biology Spectroscopy inflammation cytokines SPION macrophages targeted delivery magnetically assisted technologies |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 23; Pages: 15125 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms232315125 |
| Popis: | The persistence of inflammatory mediators in tissue niches significantly impacts regenerative outcomes and contributes to chronic diseases. Interleukin-4 (IL4) boosts pro-healing phenotypes in macrophages (Mφ) and triggers the activation of signal transducer and activator of transcription 6 (STAT6). Since the IL4/STAT6 pathway reduces Mφ responsiveness to inflammation in a targeted and precise manner, IL4 delivery offers personalized possibilities to overcome inflammatory events. Despite its therapeutic potential, the limited success of IL4-targeted delivery is hampered by inefficient vehicles. Magnetically assisted technologies offer precise and tunable nanodevices for the delivery of cytokines by combining contactless modulation, high tissue penetration, imaging features, and low interference with the biological environment. Although superparamagnetic iron oxide nanoparticles (SPION) have shown clinical applicability in imaging, SPION-based approaches have rarely been explored for targeted delivery and cell programming. Herein, we hypothesized that SPION-based carriers assist in efficient IL4 delivery to Mφ, favoring a pro-regenerative phenotype (M2φ). Our results confirmed the efficiency of SPION-IL4 and Mφ responsiveness to SPION-IL4 with evidence of STAT6-mediated polarization. SPION-IL4-treated Mφ showed increased expression of M2φ associated-mediators (IL10, ARG1, CCL2, IL1Ra) when compared to the well-established soluble IL4. The ability of SPION-IL4 to direct Mφ polarization using sophisticated magnetic nanotools is valuable for resolving inflammation and assisting innovative strategies for chronic inflammatory conditions. |
| Databáze: | OpenAIRE |
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