Pore-forming toxins: Experiments with S. aureus α-toxin, C. perfringens θ-toxin and E. coli haemolysin in lipid bilayers, liposomes and intact cells
| Autor: | C. L. Bashford, Gianfranco Menestrina, Charles A. Pasternak |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Staphylococcus aureus
Cations Divalent Clostridium perfringens Bacterial Toxins Lipid Bilayers Phospholipid Sulfur Radioisotopes Toxicology medicine.disease_cause Ion Channels Divalent Hemolysin Proteins Mice chemistry.chemical_compound Escherichia coli medicine Animals Lipid bilayer Phospholipids chemistry.chemical_classification Pore-forming toxin Liposome L-Lactate Dehydrogenase Cell Membrane Osmolar Concentration Membrane Proteins Proteins Dextrans Hemolysin Hydrogen-Ion Concentration Fluoresceins Calcein chemistry Biochemistry Type C Phospholipases Liposomes |
| Zdroj: | Toxicon. 28:477-491 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(90)90292-f |
| Popis: | Three quite different bacterial toxins (S. aureus alpha-toxin, C. perfringens theta-toxin and E. coli haemolysin) induce the leakage of phosphorylated metabolites from Lettre cells and of calcein from liposomes; in each case leakage is inhibited by Zn2+ greater than Ca2+ greater than Mg2+. Inhibition is not due to displacement of toxin from the membrane, since divalent cations inhibit leakage through pre-formed pores. Electrical conductivity across phospholipid bilayers is induced by each of the three toxins; in each case the probability of channels being in the open state is reduced by divalent cations. Although the pores induced in phospholipid bilayers and liposomes vary greatly in size (theta-toxin much greater than haemolysin greater than alpha-toxin), in Lettre cells the lesions appear more uniform, suggestive of a limiting effect in cells. |
| Databáze: | OpenAIRE |
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