Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria
| Autor: | Elena Caro, Vicente Medina, Changfu Zhu, Xi Jiang, Ana Pérez-González, Luis M. Rubio, Álvaro Eseverri, Can Baysal, Paul Christou |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Signal peptide Mitochondrial protein Nuclear gene Biología Saccharomyces cerevisiae Amino Acid Motifs Green Fluorescent Proteins Arabidopsis Mitochondrion Protein Sorting Signals medicine.disease_cause 01 natural sciences Neurospora crassa Protein sorting 03 medical and health sciences Protein targeting Tobacco Genetics medicine Arabidopsis thaliana Green fluorescent protein Phylogeny Plant Proteins Cell Nucleus Original Paper Oryza sativa biology Subcellular targeting Mitochondrial pre-sequence fungi food and beverages Oryza biology.organism_classification Peptide Fragments Recombinant Proteins Cell biology Mitochondria Protein Transport 030104 developmental biology Medio Ambiente Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Transgenic Research, ISSN 0962-8819, 2020-04, Vol. 29, No. 2 Archivo Digital UPM Universidad Politécnica de Madrid Recercat. Dipósit de la Recerca de Catalunya instname Transgenic Research Repositorio Abierto de la UdL Universitad de Lleida |
| Popis: | Mitochondria fulfil essential functions in respiration and metabolism as well as regulating stress responses and apoptosis. Most native mitochondrial proteins are encoded by nuclear genes and are imported into mitochondria via one of several receptors that recognize N-terminal signal peptides. The targeting of recombinant proteins to mitochondria therefore requires the presence of an appropriate N-terminal peptide, but little is known about mitochondrial import in monocotyledonous plants such as rice (Oryza sativa). To gain insight into this phenomenon, we targeted nuclear-encoded enhanced green fluorescent protein (eGFP) to rice mitochondria using six mitochondrial pre-sequences with diverse phylogenetic origins, and investigated their effectiveness by immunoblot analysis as well as confocal and electron microscopy. We found that the ATPA and COX4 (Saccharomyces cerevisiae), SU9 (Neurospora crassa), pFA (Arabidopsis thaliana) and OsSCSb (Oryza sativa) peptides successfully directed most of the eGFP to the mitochondria, whereas the MTS2 peptide (Nicotiana plumbaginifolia) showed little or no evidence of targeting ability even though it is a native plant sequence. Our data therefore indicate that the presence of particular recognition motifs may be required for mitochondrial targeting, whereas the phylogenetic origin of the pre-sequences probably does not play a key role in the success of mitochondrial targeting in dedifferentiated rice callus and plants. |
| Databáze: | OpenAIRE |
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