MiR-124 is differentially expressed in derivatives of the sympathoadrenal cell lineage and promotes neurite elongation in chromaffin cells
| Autor: | Hermann Rohrer, Priyanka Narasimhan, Tehani El Faitwri, Jutta Stubbusch, Uwe Ernsberger, Klaus Unsicker, Katrin Huber, Stella Shtukmaster |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell type Sympathetic Nervous System Histology Neurite Pyridines Chromaffin Cells Enteroendocrine cell Biology PC12 Cells Pathology and Forensic Medicine Mice 03 medical and health sciences 0302 clinical medicine Neurites medicine Animals Cell Lineage Nerve Growth Factors Protein Kinase Inhibitors In Situ Hybridization Gene Expression Profiling Cell Biology Transfection Amides Rats Up-Regulation MicroRNAs 030104 developmental biology medicine.anatomical_structure Nerve growth factor nervous system Adrenal Medulla Chromaffin cell biology.protein Adrenal medulla Neuroscience 030217 neurology & neurosurgery Neurotrophin |
| Zdroj: | Cell and Tissue Research. 365:225-232 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-016-2395-9 |
| Popis: | The neural-crest-derived sympathoadrenal cell lineage gives rise to sympathetic neurons and to endocrine chromaffin cells of the adrenal medulla. Both cell types express a largely overlapping set of genes, including those coding for the molecular machinery related to the synthesis and exocytotic release of catecholamines. During their early development, sympathetic neurons and chromaffin cells rely on a shared transcription factor network that controls the establishment of these common features. Despite many similarities, mature sympathetic neurons and chromaffin cells significantly differ regarding their morphology and function. Most prominently, sympathetic neurons possess axons that are absent in mammalian adrenal chromaffin cells. The molecular mechanism underlying the divergent development of sympathoadrenal cells into neuronal and endocrine cells remains elusive. Mutational inactivation of the ribonuclease dicer hints at the importance of microRNAs in this diversification. We show here that miR-124 is detectable in developing sympathetic neurons but absent in chromaffin cell precursors. We further demonstrate that miR-124 promotes neurite elongation when transfected into cultured chromaffin cells indicating its capability to support the establishment of a neuronal morphology in non-neuronal sympathoadrenal cells. Our results also show that treatment of PC12 cells with the neurotrophin nerve growth factor leads to an upregulation of miR-124 expression and that inhibition of miR-124 reduces nerve-growth-factor-induced neurite outgrowth in PC12 cells. Thus, our data indicate that miR-124 contributes to the establishment of specific neuronal features in developing sympathoadrenal cells. |
| Databáze: | OpenAIRE |
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