Evolution and functional diversification of fructose bisphosphate aldolase genes in photosynthetic marine diatoms
| Autor: | Angelika Eckert, Chris Bowler, Ahmed A. Moustafa, Andrew E. Allen, Anton Montsant, Peter G. Kroth |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
carbon metabolism Recombinant Fusion Proteins carbonic anhydrase 01 natural sciences Pyrenoid Evolution Molecular 03 medical and health sciences Phylogenetics Fructose-Bisphosphate Aldolase ddc:570 Botany Genetics Gene family 14. Life underwater Phaeodactylum tricornutum Plastids Plastid Molecular Biology Gene Ecology Evolution Behavior and Systematics Research Articles Phylogeny 030304 developmental biology Carbonic Anhydrases Diatoms 0303 health sciences biology carbon concentrating mechanism (CCM) fungi biology.organism_classification diatom Carbon Chloroplast fructose bisphosphate aldolase Diatom pyrenoid Evolutionary biology Phytoplankton 010606 plant biology & botany |
| Zdroj: | Molecular Biology and Evolution |
| ISSN: | 0737-4038 |
| DOI: | 10.1093/molbev/msr223 |
| Popis: | Diatoms and other chlorophyll-c containing, or chromalveolate, algae are among the most productive and diverse phytoplankton in the ocean. Evolutionarily, chlorophyll-c algae are linked through common, although not necessarily monophyletic, acquisition of plastid endosymbionts of red as well as most likely green algal origin. There is also strong evidence for a relatively high level of lineage-specific bacterial gene acquisition within chromalveolates. Therefore, analyses of gene content and derivation in chromalveolate taxa have indicated particularly diverse origins of their overall gene repertoire. As a single group of functionally related enzymes spanning two distinct gene families, fructose 1,6-bisphosphate aldolases (FBAs) illustrate the influence on core biochemical pathways of specific evolutionary associations among diatoms and other chromalveolates with various plastid-bearing and bacterial endosymbionts. Protein localization and activity, gene expression, and phylogenetic analyses indicate that the pennate diatom Phaeodactylum tricornutum contains five FBA genes with very little overall functional overlap. Three P. tricornutum FBAs, one class I and two class II, are plastid localized, and each appears to have a distinct evolutionary origin as well as function. Class I plastid FBA appears to have been acquired by chromalveolates from a red algal endosymbiont, whereas one copy of class II plastid FBA is likely to have originated from an ancient green algal endosymbiont. The other copy appears to be the result of a chromalveolate-specific gene duplication. Plastid FBA I and chromalveolate-specific class II plastid FBA are localized in the pyrenoid region of the chloroplast where they are associated with β-carbonic anhydrase, which is known to play a significant role in regulation of the diatom carbon concentrating mechanism. The two pyrenoid-associated FBAs are distinguished by contrasting gene expression profiles under nutrient limiting compared with optimal CO2 fixation conditions, suggestive of a distinct specialized function for each. Cytosolically localized FBAs in P. tricornutum likely play a role in glycolysis and cytoskeleton function and seem to have originated from the stramenopile host cell and from diatom-specific bacterial gene transfer, respectively. published |
| Databáze: | OpenAIRE |
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