Optimization of antioxidant, anti-diabetic, and anti-inflammatory activities and ganoderic acid content of differentially dried Ganoderma lucidum using response surface methodology
Autor: | Da Hye Ryu, Jwa Yeong Cho, Bokyung Lee, Nooruddin Bin Sadiq, Jin-Chul Kim, Muhammad Hamayun, Chu Won Nho, Ho-Youn Kim, Taek Sung Lee, Hyoung Seok Kim, Soo Hyun Park |
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Rok vydání: | 2021 |
Předmět: |
Reishi
Antioxidant medicine.drug_class medicine.medical_treatment Anti-Inflammatory Agents Chemical Fractionation Polysaccharide 01 natural sciences Antioxidants Anti-inflammatory Cell Line Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Polysaccharides medicine Humans Hypoglycemic Agents Food science Response surface methodology Ganoderma lucidum chemistry.chemical_classification Plant Extracts 010401 analytical chemistry Extraction (chemistry) Ganoderic acid 04 agricultural and veterinary sciences General Medicine 040401 food science Triterpenes 0104 chemical sciences HaCaT chemistry Food Science |
Zdroj: | Food Chemistry. 335:127645 |
ISSN: | 0308-8146 |
DOI: | 10.1016/j.foodchem.2020.127645 |
Popis: | The dried Ganoderma lucidum (GL) has been widely used for its pharmacological properties and bioactive ganoderic acids (GAs). Herein, extraction procedures combining ultra-sonication and heating were optimized using response surface methodology based on four variables (antioxidant activity, anti-diabetic activity, total GAs content, and total polysaccharide content) and principal component analysis. The extraction of freeze-dried GL at temperatures between 64.2 and 70 °C for 1.2 h maximized the antioxidant activity and GA content, whereas the polysaccharide content and anti-diabetic activity were maximized by extraction between 66.8 and 70 °C for more than 2.8 h. Heat-dried GL extracted at 50 °C for 3 h provided the greatest anti-inflammatory activity against HaCaT cells by suppressing the response to inflammation related cytokines at mRNA levels. These results suggest that extraction conditions might be a limiting factor for target-oriented investigations, and optimized extraction methods may improve the potential effect and quality of harvested GL products. |
Databáze: | OpenAIRE |
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