Altered PI3-kinase/Akt signalling in skeletal muscle of young men with low birth weight
| Autor: | Malgorzata S. Martin-Gronert, Heidi Storgaard, Susan E. Ozanne, Christine B. Jensen, Sten Madsbad, Allan Vaag |
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| Přispěvatelé: | Ozanne, Susan [0000-0001-8753-5144], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty MAP Kinase Signaling System medicine.medical_treatment lcsh:Medicine Type 2 diabetes Cell Biology/Cell Signaling Phosphatidylinositol 3-Kinases Young Adult Insulin resistance Internal medicine medicine Humans Insulin Diabetes and Endocrinology/Type 2 Diabetes Phosphorylation lcsh:Science Muscle Skeletal Protein kinase B Protein Kinase C Multidisciplinary Glucose Transporter Type 4 biology lcsh:R Infant Newborn Skeletal muscle Fasting Infant Low Birth Weight medicine.disease Nutrition/Malnutrition IRS1 Insulin receptor medicine.anatomical_structure Endocrinology biology.protein Insulin Receptor Substrate Proteins lcsh:Q Proto-Oncogene Proteins c-akt GLUT4 Research Article Signal Transduction |
| Zdroj: | PLoS ONE PLoS ONE, Vol 3, Iss 11, p e3738 (2008) |
| DOI: | 10.17863/cam.56183 |
| Popis: | BACKGROUND: Low birth weight (LBW) is associated with increased future risk of insulin resistance and type 2 diabetes mellitus. The underlying molecular mechanisms remain poorly understood. We have previously shown that young LBW men have reduced skeletal muscle expression of PI3K p85alpha regulatory subunit and p110beta catalytic subunit, PKCzeta and GLUT4 in the fasting state. The aim of this study was to determine whether insulin activation of the PI3K/Akt and MAPK signalling pathways is altered in skeletal muscle of young adult men with LBW. METHODS: Vastus lateralis muscle biopsies were obtained from 20 healthy 19-yr old men with BW< or = 10th percentile for gestational age (LBW) and 20 normal birth weight controls (NBW), matched for physical fitness and whole-body glucose disposal, prior to (fasting state) and following a 4-hr hyperinsulinemic euglycemic clamp (insulin stimulated state). Expression and phosphorylation of selected proteins was determined by Western blotting. PRINCIPAL FINDINGS: Insulin stimulated expression of aPKCzeta (p |
| Databáze: | OpenAIRE |
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