IGF-I is required for normal embryonic growth in mice
| Autor: | Lyn Powell-Braxton, P Hollingshead, Sharon Pitts-Meek, M Dowd, C Warburton, D Dalton, Timothy A. Stewart, N Gillett |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Muscle tissue medicine.medical_specialty Ratón medicine.medical_treatment Molecular Sequence Data Biology Carbohydrate metabolism Cell Line Embryonic and Fetal Development Mice Pregnancy Internal medicine Genetics medicine Animals Insulin-Like Growth Factor I Base Sequence Growth factor Embryogenesis Wild type Mice Inbred C57BL Phenotype Endocrinology medicine.anatomical_structure Female Homeostasis Developmental Biology Hormone |
| Zdroj: | Genes & Development. 7:2609-2617 |
| ISSN: | 0890-9369 |
| DOI: | 10.1101/gad.7.12b.2609 |
| Popis: | IGF-I is a pleiotropic hormone reported to affect linear growth, glucose metabolism, organ homeostasis, and the immune and neurologic systems. In contrast to IGF-II, IGF-I is expressed at low levels embryonically and has been thought to be more important for postnatal growth and development. To investigate the role of IGF-I in normal development we generated mice with an inactive IGF-I gene by homologous recombination in ES cells. Heterozygous mice are healthy and fertile, but they are 10-20% smaller than wild-type littermates and have lower than normal levels of IGF-I. The size reduction is attributable to a decrease in organs and muscle and bone mass. However, all tissues appear histologically normal. At birth homozygous mutant mice (IGF-I-/-) are < 60% body weight of wild type. Greater than 95% of IGF-I-/- pups die perinatally. Histopathology is characterized by underdevelopment of muscle tissue. Lungs of late embryonic and neonates also appeared less organized with ill-defined alveolae. IGF-I appears to be essential for correct embryonic development in mice. |
| Databáze: | OpenAIRE |
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