Comparative ability of chicken and mammalian LHRH to release LH from rooster pituitary cells
| Autor: | Alan L. Johnson, R. W. Dickerman, J. P. Advis |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Rooster In Vitro Techniques Peptide hormone High-performance liquid chromatography General Biochemistry Genetics and Molecular Biology Gonadotropin-Releasing Hormone Biological property Internal medicine medicine Animals General Pharmacology Toxicology and Pharmaceutics Incubation Chromatography High Pressure Liquid Mammals Dose-Response Relationship Drug biology Phosphate buffered saline General Medicine Luteinizing Hormone biology.organism_classification In vitro Endocrinology Pituitary Gland Chickens Retention time hormones hormone substitutes and hormone antagonists |
| Zdroj: | Life Sciences. 34:1847-1851 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(84)90678-7 |
| Popis: | Biological properties of homogeneous solutions of chicken (c) and mammalian (m) LHRH were compared by their ability to release LH, in vitro, from a rooster pituitary cell incubation system. Homogeneity of the two LHRH species was confirmed by High Performance Liquid Chromatography (HPLC) using linear gradients of acetonitrile and phosphate buffer. A clear HPLC separation of [Gln8]-LHRH ( cLHRH ) and [Arg8]-LHRH ( mLHRH ) was obtained, with the former having a consistently longer retention time than the latter. cLHRH cause a greater (p less than .025) in vitro release of LH at low doses (less than 1 ng/2 X 10(5) live pituitary cells), but not at high doses (greater than 10 ng/2 X 10(5) live pituitary cells), than that caused by mLHRH . Our results indicate that rooster pituitary cells are significantly more sensitive to low doses of cLHRH than to similar doses of mLHRH , when assessed by their ability to release LH in vitro. |
| Databáze: | OpenAIRE |
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