EICOSAPENTAENOIC ACID ENHANCES HEATSTROKE-IMPAIRED INTESTINAL EPITHELIAL BARRIER FUNCTION IN RATS
| Autor: | Xiaowen Qiu, Fangfang Yuan, Yali Zhang, Guizhen Xiao, Yan Geng, Zhifeng Liu, Lei Su, Jiefu Lu, Li-Qun Tang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Docosahexaenoic Acids Heat Stroke Drug Evaluation Preclinical Biology Critical Care and Intensive Care Medicine Permeability Tight Junctions Ileum Internal medicine medicine Animals Intestinal Mucosa Rats Wistar chemistry.chemical_classification Tight Junction Proteins Cell Membrane Fatty Acids Heatstroke Intestinal mucosal barrier medicine.disease Eicosapentaenoic acid Endotoxins Intestines Microscopy Electron Endocrinology Eicosapentaenoic Acid Intestinal Absorption Biochemistry chemistry Docosahexaenoic acid Emergency Medicine Epithelial barrier function lipids (amino acids peptides and proteins) Polyunsaturated fatty acid |
| Zdroj: | Shock. 44:348-356 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/shk.0000000000000417 |
| Popis: | Dysfunction of the intestinal barrier plays an important role in the pathological process of heatstroke. Omega-3 (or n-3) polyunsaturated fatty acids, including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), help protect the intestinal mucosal barrier. This study assessed if pretreating rats with EPA or DHA could alleviate heat stress-induced damage to the intestinal barrier caused by experimental heatstroke.Male Wistar rats were pregavaged with either EPA, DHA, corn oil, or normal saline (all 1 g/kg) for 21 days before the heatstroke experiment (control rats were not exposed to heat). Experimental rats were exposed to an ambient temperature of 37°C and 60% humidity to induce heatstroke, and then they were allowed to recover at room temperature after rapid cooling. Survival time of rats was monitored after heatstroke. Horseradish peroxidase flux from the gut lumen and the level of plasma D-lactate were measured to analyze intestinal permeability at 6 h after heatstroke. Plasma endotoxin levels were determined using a limulus amoebocyte lysate assay. Expressions of the tight junction (TJ) proteins occludin and ZO-1 were analyzed by Western blot and localized by immunofluorescence microscopy. Tight junction protein morphology was observed by transmission electron microscopy. Fatty acids of ileal mucosa were analyzed using gas chromatography-mass selective detector.Eicosapentaenoic acid significantly increased survival time after heatstroke. Eicosapentaenoic acid significantly decreased intestinal permeability and plasma endotoxin levels. Eicosapentaenoic acid effectively attenuated the heatstroke-induced disruption of the intestinal structure and improved the histology score, whereas DHA was less effective, and corn oil was ineffective. Pretreatment with EPA also increased expression of occludin and ZO-1 to effectively prevent TJ disruption. Eicosapentaenoic acid pretreatment enriched itself in the membrane of intestinal cells.Our results indicate that EPA pretreatment is more effective than DHA pretreatment in attenuating heat-induced intestinal dysfunction and preventing TJ damage. Enhanced expression of TJ proteins that support the epithelial barrier integrity may be important for maintaining a functional intestinal barrier during heatstroke. |
| Databáze: | OpenAIRE |
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