Phylogeography of the heavily poached African common pangolin (Pholidota, Manis tricuspis ) reveals six cryptic lineages as traceable signatures of Pleistocene diversification

Autor: Chabi A.M.S. Djagoun, William T. Stanley, Komlan Mawuli Afiademanyo, Flobert Njiokou, Jean Malekani, Christelle Tougard, Philippe Gaubert, Agostinho Antunes, Sery Gonedele Bi, Prince Kaleme, Emmanuel Danquah, Sylvain Dufour, Gabriel Ngua, Ayodeji Olayemi, Shu-Jin Luo, Casimir Nebesse Mololo
Přispěvatelé: Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UR226, Laboratoire de Parasitologie et Ecologie [Youndé], Université de Yaoundé I, ANDEGE, Departement de Zoologie et de Biologie Animale, SYLVATROP, Department of Biology, University of Kinshasa, Obafemi Awolowo University (OAU), Natural History Museum, Obafemi Awolowo University, Department of Wildlife and Range Management, Kwame Nkrumah University of Science and Technology [GHANA] (KNUST), Laboratory of Applied Ecology, Laboratoire de Mammalogie, Musée royal de l'Afrique centrale, Université de Kisangani, School of Life Sciences, Peking- Tsinghua Center for Life Sciences, Departamento de Biologia, Faculdade de Ciências da Universidade do Porto, Universidade do Porto
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Mitochondrial DNA
evolutionary significant units
Rainforest
[SDV.BID]Life Sciences [q-bio]/Biodiversity
Biology
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics
Phylogenetics and taxonomy
DNA
Mitochondrial
Ghana
010603 evolutionary biology
01 natural sciences
Evolution
Molecular
03 medical and health sciences
Genetics
molecular tracing
Animals
Gabon
Phylogeny
Ecology
Evolution
Behavior and Systematics
trade monitoring
Phylogenetic tree
tropical Africa
Eutheria
Cytochrome b
Ecology
[SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE]
Pangolin
Pholidota
Bayes Theorem
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
15. Life on land
pangolins
biology.organism_classification
Phylogeography
030104 developmental biology
Evolutionary biology
Manis tricuspis
Pleistocene diversification
Zdroj: Molecular Ecology
Molecular Ecology, Wiley, 2016, 25 (23), pp.5975-5993. ⟨10.1111/mec.13886⟩
ISSN: 0962-1083
1365-294X
Popis: Knowledge on faunal diversification in African rainforests remains scarce. We used phylogeography to assess (i) the role of Pleistocene climatic oscillations in the diversification of the African common pangolin (Manis tricuspis), and (ii) the utility of our multi-locus approach for taxonomic delineation and trade tracing of this heavily poached species. We sequenced 101 individuals for two mitochondrial DNA (mtDNA), two nuclear DNA and one Y-borne gene fragments (totalizing 2,602 bp). We used a time-calibrated, Bayesian inference phylogenetic framework, and conducted character-based, genetic and phylogenetic delineation of species hypotheses within African common pangolins. We identified six geographic lineages partitioned into western Africa, Ghana, the Dahomey Gap, western central Africa, Gabon and central Africa, all diverging during the Middle to Late Pleistocene. MtDNA (cytochrome b + control region) was the sole locus to provide diagnostic characters for each of the six lineages. Tree-based Bayesian delimitation methods using single- and multi-locus approaches gave high support for ‘species’ level recognition of the six African common pangolin lineages. Although the diversification of African common pangolins occurred during Pleistocene cyclical glaciations, causative correlation with traditional rainforest refugia and riverine barriers in Africa was not straightforward. We conclude on the existence of six cryptic lineages within African common pangolins, which might be of major relevance for future conservation strategies. The high discriminative power of the mtDNA markers used in this study should allow an efficient molecular tracing of the regional origin of African common pangolin seizures. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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