A Multifaceted Role for Myd88-Dependent Signaling in Progression of Murine Mammary Carcinoma
| Autor: | Maureen A. Carey, Tiffany Phuong, Antonio Serrano, Alyssa Seifert, Kyle C. Tucker, Victoria A. Parsons, Mary Higgins, Alec Eidelman, Robert A. Kurt, Kofi Y. Boateng, Jacob M. Ricca |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway Cancer Research Chemokine CCL2 4T1 Myd88 Bioinformatics lcsh:RC254-282 03 medical and health sciences breast cancer 0302 clinical medicine Medicine RNA-Seq Receptor STAT5 Original Research biology Kinase business.industry lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology Oncology Tumor progression 030220 oncology & carcinogenesis biology.protein STAT protein Cancer research business NFκB |
| Zdroj: | Breast Cancer: Basic and Clinical Research, Vol 10 (2016) Breast Cancer: Basic and Clinical Research, Vol 2016, Iss 10, Pp 157-167 (2016) Breast Cancer : Basic and Clinical Research |
| ISSN: | 1178-2234 |
| Popis: | Previous data obtained in our laboratory suggested that there may be constitutive signaling through the myeloid differentiation primary response gene 88 (Myd88)-dependent signaling cascade in murine mammary carcinoma. Here, we extended these findings by showing that, in the absence of an added Toll-like receptor (TLR) agonist, the myddosome complex was preformed in 4T1 tumor cells, and that Myd88 influenced cytoplasmic extracellular signal–regulated kinase (Erk)1/Erk2 levels, nuclear levels of nuclear factor-kappaB (NFκB) and signal transducer and activator of transcription 5 (STAT5), tumor-derived chemokine (C–C motif) ligand 2 (CCL2) expression, and in vitro and in vivo tumor growth. In addition, RNA-sequencing revealed that Myd88-dependent signaling enhanced the expression of genes that could contribute to breast cancer progression and genes previously associated with poor outcome for patients with breast cancer, in addition to suppressing the expression of genes capable of inhibiting breast cancer progression. Yet, Myd88-dependent signaling in tumor cells also suppressed expression of genes that could contribute to tumor progression. Collectively, these data revealed a multifaceted role for Myd88-dependent signaling in murine mammary carcinoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|