Adenosine A1, A2a, A2b, and A3 Receptors in Hematopoiesis. 2. Expression of Receptor mRNA in Resting and LipopolysaccharideActivated Mouse RAW 264.7 Macrophages
| Autor: | D. Streitova, Michal Hofer, Milan Pospíšil, J. Holá, Antonín Vacek |
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| Rok vydání: | 2010 |
| Předmět: |
Lipopolysaccharides
Time Factors Receptor Adenosine A2A Lipopolysaccharide Physiology Biology Receptor Adenosine A2B Polymerase Chain Reaction Cell Line Mice chemistry.chemical_compound Downregulation and upregulation Animals RNA Messenger Receptor Messenger RNA Receptor Adenosine A1 Macrophages Receptor Adenosine A3 Receptors Purinergic P1 General Medicine Macrophage Activation Purinergic signalling Adenosine A3 receptor Adenosine receptor Molecular biology Hematopoiesis Gene Expression Regulation chemistry Adenosine A2B receptor |
| Zdroj: | Physiological Research. :139-144 |
| ISSN: | 1802-9973 0862-8408 |
| DOI: | 10.33549/physiolres.931724 |
| Popis: | Expression of mRNA for adenosine receptor subtypes A(1), A(2a), A(2b), and A(3) in normal and lipopolysaccharide (LPS)-activated murine RAW 264.7 macrophages has been investigated using the method of quantitative real-time polymerase chain reaction. The results have shown a very low, unquantifiable expression of adenosine A(1) receptor mRNA in both normal and LPS-activated macrophages. The other three adenosine receptor mRNAs have been found to be expressed at various but always quantifiable levels. Activation of the macrophages by LPS induced upregulation of the expression of adenosine receptor A(2a) and A(2b) mRNA, whereas the expression of adenosine receptor A(3) mRNA was downregulated. Unstimulated macrophages exhibited a high expression of the A(2b) adenosine receptor mRNA. The findings are discussed from the point of view of the antiinflammatory and hematopoiesis-stimulating roles of the adenosine receptor signaling. |
| Databáze: | OpenAIRE |
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