Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body
| Autor: | Che-Chia Tsao, Martin A. Gorovsky |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Genotype
Blotting Western Cell Protozoan Proteins Biology Models Biological Polymerase Chain Reaction Open Reading Frames Intraflagellar transport medicine Animals Basal body Cilia Ciliary tip Undulipodium Cilium Regeneration (biology) Tetrahymena Cell Biology Blotting Northern biology.organism_classification Cell biology Protein Transport medicine.anatomical_structure Mutation sense organs |
| Zdroj: | Journal of Cell Science. 121:428-436 |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | Intraflagellar transport (IFT) moves multiple protein particles composed of two biochemically distinct complexes, IFT-A and IFT-B, bi-directionally within cilia and is essential for cilia assembly and maintenance. We identified an ORF from the Tetrahymena macronuclear genome sequence, encoding IFT122A, an ortholog of an IFT-A complex protein. Tetrahymena IFT122A is induced during cilia regeneration, and epitope-tagged Ift122Ap could be detected in isolated cilia. IFT122A knockout cells still assembled cilia, albeit with lower efficiency, and could regenerate amputated cilia. Ift172p and Ift88p, two IFT-B complex proteins that localized mainly to basal bodies and along the cilia in wild-type cells, became preferentially enriched at the ciliary tips in IFT122A knockout cells. Our results indicate that Tetrahymena IFT122A is not required for anterograde transport-dependent ciliary assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body. |
| Databáze: | OpenAIRE |
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