Covalent Defects Restrict Supramolecular Self-Assembly of Homopolypeptides: Case Study of β2-Fibrils of Poly-L-Glutamic Acid

Autor: Wojciech Dzwolak, Agnieszka Hernik, Hanna Nieznanska, Aleksandra Fulara
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Protein Structure
Amyloid
Biosynthetic Techniques
Supramolecular chemistry
Biophysics
Infrared spectroscopy
lcsh:Medicine
Context (language use)
Synthetic Biotechnology
Bioinformatics
Research and Analysis Methods
Biochemistry
Physical Chemistry
chemistry.chemical_compound
Polylactic Acid-Polyglycolic Acid Copolymer
Side chain
Synthetic Peptides
Lactic Acid
lcsh:Science
Synthetic Polypeptides
Peptide Synthesis
Multidisciplinary
Chemical Bonding
Chemistry
Hydrogen bond
Physics
lcsh:R
Biology and Life Sciences
Proteins
Chemical Synthesis
Hydrogen Bonding
Polymer Chemistry
Protein Aggregation
Crystallography
PLGA
Macromolecules
Polyglutamic Acid
Covalent bond
Bionanotechnology
Physical Sciences
Engineering and Technology
lcsh:Q
Synthetic Biology
Self-assembly
Peptides
Polyglycolic Acid
Research Article
Biotechnology
Zdroj: PLoS ONE
PLoS ONE, Vol 9, Iss 8, p e105660 (2014)
ISSN: 1932-6203
Popis: Poly-L-glutamic acid (PLGA) often serves as a model in studies on amyloid fibrils and conformational transitions in proteins, and as a precursor for synthetic biomaterials. Aggregation of PLGA chains and formation of amyloid-like fibrils was shown to continue on higher levels of superstructural self-assembly coinciding with the appearance of so-called β2-sheet conformation manifesting in dramatic redshift of infrared amide I' band below 1600 cm(-1). This spectral hallmark has been attributed to network of bifurcated hydrogen bonds coupling C = O and N-D (N-H) groups of the main chains to glutamate side chains. However, other authors reported that, under essentially identical conditions, PLGA forms the conventional in terms of infrared characteristics β1-sheet structure (exciton-split amide I' band with peaks at ca. 1616 and 1683 cm(-1)). Here we attempt to shed light on this discrepancy by studying the effect of increasing concentration of intentionally induced defects in PLGA on the tendency to form β1/β2-type aggregates using infrared spectroscopy. We have employed carbodiimide-mediated covalent modification of Glu side chains with n-butylamine (NBA), as well as electrostatics-driven inclusion of polylysine chains, as two different ways to trigger structural defects in PLGA. Our study depicts a clear correlation between concentration of defects in PLGA and increasing tendency to depart from the β2-structure toward the one less demanding in terms of chemical uniformity of side chains: β1-structure. The varying predisposition to form β1- or β2-type aggregates assessed by infrared absorption was compared with the degree of morphological order observed in electron microscopy images. Our results are discussed in the context of latent covalent defects in homopolypeptides (especially with side chains capable of hydrogen-bonding) that could obscure their actual propensities to adopt different conformations, and limit applications in the field of synthetic biomaterials.
Databáze: OpenAIRE
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