Binding Affinity of Concanavalin A to Native and Acid-Hydrolyzed Phytoglycogen Nanoparticles

Autor:	Kathleen Charlesworth, Nicholas van Heijst, Aidan Maxwell, Benjamin Baylis, Michael Grossutti, J. Jay Leitch, John R. Dutcher
Rok vydání:	2022
Předmět:	Biomaterials Spectrophotometry Infrared Polymers and Plastics Concanavalin A Materials Chemistry Nanoparticles Proteins Bioengineering Surface Plasmon Resonance
Zdroj:	Biomacromolecules. 23:4778-4785
ISSN:	1526-4602 1525-7797
Popis:	Phytoglycogen (PG) is a polysaccharide produced in the kernels of sweet corn as soft, highly branched, compact nanoparticles. Its tree-like or dendritic architecture, combined with a high-safety profile, makes PG nanoparticles attractive for use in biological applications, many of which rely on the association or binding of small biomolecules. We have developed a methodology to functionalize surface plasmon resonance (SPR) sensor surfaces with PG nanoparticles, and we demonstrate the utility of the PG-functionalized SPR sensor by measuring the binding affinity of the tetrameric concanavalin A (ConA) protein to both native PG nanoparticles and smaller, softer acid-hydrolyzed PG nanoparticles. We measure comparable values of the equilibrium association constant
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c0d0a8c3200512eaca82387c39f3781 https://doi.org/10.1021/acs.biomac.2c00951 Zobrazit plný text záznamu