Hepatic Tissue Engineering on 3-Dimensional Biodegradable Polymers within a Pulsatile Flow Bioreactor
| Autor: | Jörg Petersen, C. Vogel, Eva Török, Martin R. Burda, Xavier Rogiers, J. M. Pollok, Dietrich Kluth, Peter Kaufmann, Peter X. Ma, Maura Dandri |
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| Rok vydání: | 2001 |
| Předmět: |
Time Factors
Cell Transplantation Polymers Biomedical Engineering Pulsatile flow Biocompatible Materials In Vitro Techniques Bioreactors Hepatocyte transplantation Tissue engineering Albumins Spheroids Cellular Bioreactor Animals Medicine Lactic Acid business.industry Gastroenterology Spheroid Hepatic tissue Anatomy Biodegradable polymer Liver Transplantation Rats Rats Inbred Lew Pulsatile Flow Hepatocytes Surgery business Biomedical engineering |
| Zdroj: | Digestive Surgery. 18:196-203 |
| ISSN: | 1421-9883 0253-4886 |
| Popis: | Background: An optimal method for hepatocyte transplantation is not yet determined. With the principles of tissue engineering in vitro conditioning of hepatocytes on biodegradable polymer in a flow bioreactor before implantation forming spheroids may achieve increased cell mass and function to replace lost organ function in vivo. Methods: Biodegradable poly-L-lactic (PLLA) polymer discs were seeded with rat hepatocytes in a concentration of 10 × 106 cells per ml and exposed to a medium flow of 24 ml/min for 1, 2, 4 and 6 days. The number and diameter of spheroidal aggregates was measured by phase-contrast microscopy. H&E histology was performed. Albumin production as hepatocyte specific function was determined by ELISA. Results: Spheroids of viable hepatocytes of 50–200 µm in diameter were formed. Both the number and diameter of the spheroids increased during the first 2 days and then remained constant until day 6. Albumin production was maintained throughout the culture period. Conclusion: Short (2– 3 days) pre-transplant conditioning of hepatocytes in a flow bioreactor on biodegradable PLLA resulted in formation of spheroids with a liver-like morphology and preserved specific metabolic function. Tissue engineered hepatocyte spheroids on polymer may represent a functionally active and easy transplantable neotissue and may serve as an in vivo substitute for lost liver function. |
| Databáze: | OpenAIRE |
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