Distinction of acute lymphoblastic leukemia from acute myeloid leukemia through microarray-based DNA methylation analysis
| Autor: | Bernd Dörken, Matthias Burger, Inko Nimmrich, Wolf-Dieter Ludwig, Evelyne Becker, Sabine Maier, Christian Scholz |
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| Rok vydání: | 2004 |
| Předmět: |
Microarray
Guanine Biology Diagnosis Differential chemistry.chemical_compound Precursor B-Cell Lymphoblastic Leukemia-Lymphoma Humans Promoter Regions Genetic Oligonucleotide Array Sequence Analysis Acute leukemia Base Sequence Myeloid leukemia Hematology General Medicine Methylation DNA Methylation Precursor Cell Lymphoblastic Leukemia-Lymphoma Classification Molecular biology Neoplasm Proteins chemistry CpG site Leukemia Myeloid Acute Disease DNA methylation Cancer research Dinucleoside Phosphates Cytosine |
| Zdroj: | Annals of Hematology. 84:236-244 |
| ISSN: | 1432-0584 0939-5555 |
| Popis: | Patterns of DNA methylation are substantially altered in malignancies compared to normal tissue, with both genome-wide hypomethylation and regional increase of cytosine methylation at dinucleotides of cytosine and guanine, i.e., CpG dinucleotides. While genome-wide hypomethylation renders chromosomes instable, hypermethylation of CpGs in promoter regions is generally associated with transcriptional silencing, e.g., of tumor suppressor genes. To investigate whether disease-specific methylation profiles exist for different entities of acute leukemia, a microarray-based DNA methylation analysis simultaneously assessing 249 CpG dinucleotides originating from 57 genes was employed. Hereby, samples from precursor B-cell acute lymphoblastic leukemia (ALL) could be distinguished from cases of acute myeloid leukemia by virtue of N33, EGR4, CDC2, CCND2, or MOS hypermethylation in ALL. |
| Databáze: | OpenAIRE |
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