Occurrence of 1-methyladenosine and absence of ribothymidine in transfer ribonucleic acid of Mycobacterium smegmatis
| Autor: | Z Yamaizumi, Y Taya, T Ramakrishnan, S Noguchi, S Nishimura, B R Vani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1979 |
| Předmět: |
Adenosine
medicine.disease_cause Microbiology Methylation Mycobacterium chemistry.chemical_compound RNA Transfer Species Specificity medicine Escherichia coli Ribonuclease Molecular Biology Microbiology & Cell Biology tRNA Methyltransferases biology Mycobacterium smegmatis biology.organism_classification Molecular biology TRNA Methyltransferases RNA Bacterial Biochemistry chemistry Transfer RNA biology.protein Dihydrouridine Thymidine Research Article |
| Popis: | The minor base composition of Mycobacterium smegmatis tRNA has been studied. Thin-layer chromatographic patterns of a ribonuclease T2 digest of mycobacterial tRNA indicated the presence of appreciable amounts of 1-methyladenosine (which is commonly present only in eucaryotic tRNA), dihydrouridine, and 7-methylguanosine. Ribothymidine was absent. The S-adenosylmethionine-dependent tRNA methylases of M. smegmatis catalyzed the formation of 1-methyladenosine when Escherichia coli tRNA was used as acceptor. Similarly, E. coli extracts methylated the tRNA of M. smegmatis, forming ribothymidine. |
| Databáze: | OpenAIRE |
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