Neuromuscular Electrical Stimulation Improves Energy Substrate Metabolism and Survival in Mice With Acute Endotoxic Shock

Autor: Takayuki Irahara, Kazuo Inoue, Kosuke Otake, Satoru Murata, Norio Sato, Hiroyuki Yokota, Kaoru Koike
Jazyk: angličtina
Rok vydání: 2020
Předmět:
NMES
Male
HF
VCO2
medicine.medical_treatment
CHO
LF
neuromuscular electrical stimulation
PGC-1α
Stimulation
Critical Care and Intensive Care Medicine
carbohydrate oxidation
intensive care unit
Mice
HV
VO2
LV
Beta oxidation
fatty acid oxidation
energy substrate metabolism
Chemistry
lipopolysaccharide
Interleukin
muscle stimulation
high frequency
Shock
Septic
Emergency Medicine
medicine.symptom
low frequency
Oxidation-Reduction
medicine.medical_specialty
LPS
Intraperitoneal injection
interleukin 6
Inflammation
Carbohydrate metabolism
peroxisome proliferator-activated receptor gamma coactivator 1α
maximum rate of CO2 consumption
Gastrocnemius muscle
body weight
high voltage
Internal medicine
medicine
nutritional management
Animals
low voltage
Muscle Strength
Endotoxic shock
BW
Muscle
Skeletal
IL-6
Basic Science Aspects
Calorimetry
Indirect
Metabolism
Lipid Metabolism
Electric Stimulation
FAO
Mice
Inbred C57BL
Endocrinology
ICU
Energy Metabolism
control
maximum rate of O2 consumption
Zdroj: Shock (Augusta, Ga.)
ISSN: 1540-0514
1073-2322
Popis: This study investigated the therapeutic benefits of neuromuscular electrical stimulation (NMES). C57BL/6 mice were administered lipopolysaccharide (LPS; 20 mg/kg body weight) by intraperitoneal injection and divided into control (C) and NMES groups (n = 10–12 each). The latter received NMES to the bilateral gastrocnemius muscle for 1 h at low or high frequency (LF = 2 Hz and HF = 50 Hz, respectively) and low or high voltage (LV = 10 V and HV = 50 V, respectively). In LF–LV and LF–HV groups, NMES was performed twice and the results were compared with those for mice that received one round of NMES. Changes in energy metabolism were measured by indirect calorimetry up to 24 h; survival was evaluated up to 72 h after LPS administration; peroxisome proliferator-activated receptor gamma coactivator (PGC)-1α expression in the liver and gastrocnemius muscle was evaluated by quantitative PCR; and plasma concentration of interleukin (IL)-6 was determined by enzyme-linked immunosorbent assay. Survival was improved only in the LF–LV group with one round of NMES (P < 0.01) and the LF–HV group with two rounds of NMES (P < 0.05). Fatty acid oxidation (FAO) was slightly increased in these two groups, whereas carbohydrate oxidation (CHO) was decreased or not changed. Significant upregulation of PGC-1α in muscle as well as a decrease in plasma IL-6 level were also observed in these two groups (P < 0.05). Thus, NMES exerts therapeutic effects under conditions that induce a mild switch in energy metabolism from glucose to lipid predominant metabolism through PGC-1α upregulation and suppression of inflammation, and may be an effective early intervention even in hemodynamically unstable patients.
Databáze: OpenAIRE
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