Quantification of Chlorophyll as a Proxy for Biofilm Formation in the Cyanobacterium Synechococcus elongatus
| Autor: | Ryan Simkovsky, Susan S. Golden, Eleonora Sendersky, Rakefet Schwarz |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Synechococcus elongatus Strategy and Management macromolecular substances Cyanobacteria 01 natural sciences Industrial and Manufacturing Engineering Planktonic 03 medical and health sciences chemistry.chemical_compound Affordable and Clean Energy Botany Chlorophyll measurement Chemistry Mechanical Engineering Biofilm fungi Metals and Alloys biochemical phenomena metabolism and nutrition Plankton 030104 developmental biology Chlorophyll Sessile 010606 plant biology & botany |
| Zdroj: | Bio-protocol, vol 7, iss 14 |
| Popis: | A self-suppression mechanism of biofilm development in the cyanobacterium Synechococcus elongatus PCC 7942 was recently reported. These studies required quantification of biofilms formed by mutants impaired in the biofilm-inhibitory process. Here we describe in detail the use of chlorophyll measurements as a proxy for biomass accumulation in sessile and planktonic cells of biofilm-forming strains. These measurements allow quantification of the total biomass as estimated by chlorophyll level and representation of the extent of biofilm formation by depicting the relative fraction of chlorophyll in planktonic cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|