Factors responsible for successful HLA-DR typing of mononuclear cells from cord blood
| Autor: | Ines O. Remendado, Kantilal M. Patel, Rosalinda F. Duria, Rhonda L. Andersen, Ae Za Lee, Ruta M. Radvany, Yin S. Hsieh |
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| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Immunology Cell Separation Biochemistry Peripheral blood mononuclear cell Umbilical cord Antigen Genetics medicine HLA-DR Immunology and Allergy Humans Typing Lymphocytes Gynecology medicine.diagnostic_test business.industry Histocompatibility Testing Critical factors Histocompatibility Antigens Class II Infant Newborn General Medicine HLA-DR Antigens Fetal Blood medicine.anatomical_structure Cord blood Female business Tissue typing |
| Zdroj: | Tissue antigens. 24(4) |
| ISSN: | 0001-2815 |
| Popis: | Of 115 cord blood samples tested, both HLA-DR antigens were identified in 79% of fresh samples, 66% of older samples and 55% of freshly frozen samples. Because of weak reactions, one or two questionable antigens were present in 21% of fresh samples as compared to 34% of older samples and 45% of freshly frozen samples. Older samples showed lower cell viability, more extra reactions, and a high frequency of weak reactions. Comparison of cord blood DR antigens with parental DR antigens revealed that antigens were missed or inappropriately assigned in 5 known cases. This was more frequent with older blood samples. The critical factors in successful DR typing of cord blood samples were 1) dilution of cord blood samples with medium, 2) processing of the samples as soon as possible, and 3) overnight incubation to remove adherent cells. We are grateful to Drs. Rose Payne, Angenie-ta Biegel, Helen Bashir, Rene Duquesnoy, Helene Mervart, John Hansen, Paul Reekers, Ieke Schroeder and Paul Terasaki and NIH Serum Bank for their gifts of typing sera; the faculty of the Department of Obstetrics and Gynecology at Northwestern University and the Maternity Ward patients who permitted us to collect and use post-pregnancy sera as tissue typing reagents. We also wish to thank Mrs. Donna Wilson for expert assistance in preparing this manuscript. |
| Databáze: | OpenAIRE |
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