Chemical Vapour Deposition Graphene Field-Effect Transistors for Detection of Human Chorionic Gonadotropin Cancer Risk Biomarker
| Autor: | Paul Davey, Toby Whitley, Carrie Haslam, Shakil Awan, Emmanuel Ifeachor, Samar Damiati |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Graphene 05 social sciences 050801 communication & media studies 020206 networking & telecommunications Nanotechnology 02 engineering and technology Chemical vapor deposition Graphene field effect transistors law.invention Human chorionic gonadotropin 0508 media and communications law Blood biomarkers 0202 electrical engineering electronic engineering information engineering biochemistry Biomarker (medicine) Electrochemical biosensor Cancer risk |
| DOI: | 10.20944/preprints201712.0106.v1 |
| Popis: | We report on the development of chemical vapour deposition (CVD) based graphene field effect transistor (GFET) immunosensors for the sensitive detection of Human Chorionic Gonadotropin (hCG), a glycoprotein risk biomarker of certain cancers. The GFET sensors were fabricated on Si/SiO2 substrate using photolithography with evaporated chromium and sputtered gold contacts. GFET channels were functionalized with a linker molecule to immobile anti-hCG antibody on the surface of graphene. Binding reaction of the antibody with varying concentration levels of hCG antigen demonstrated the limit of detection of the GFET sensors to be below 1pg/mL using four-probe electrical measurements. We also show annealing can significantly improve the carrier transport properties of GFETs and shift the Dirac point (Fermi level) with reduced p-doping in back-gated measurements. The developed GFET biosensors are generic and could find applications in a broad range of medical diagnostics in addition to cancer, such as neurodegenerative (Alzheimer’s, Parkinson’s and Lewy body) and cardiovascular disorders. |
| Databáze: | OpenAIRE |
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