Huangqin-Tang Ameliorates TNBS-Induced Colitis by Regulating Effector and Regulatory CD4+ T Cells
| Autor: | Yang-Jia Lu, Xue-Bao Zheng, Zhiwei He, Ying Zou, Jian Wang, Wen-Yang Li, Zheng Wan, Congcong Ding, Zhu-Guo Wu, Guo-Liang Huang, Hong-Gang Chi, Bin-Bin Li, Bing Zhao |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Article Subject
lcsh:Medicine Decoction Inflammatory bowel disease T-Lymphocytes Regulatory General Biochemistry Genetics and Molecular Biology Rats Sprague-Dawley chemistry.chemical_compound Mesalazine Medicine Animals Huangqin-Tang Colitis Transcription factor General Immunology and Microbiology business.industry Effector lcsh:R FOXP3 General Medicine medicine.disease Rats chemistry Trinitrobenzenesulfonic Acid Immunology Cytokines Th17 Cells business Drugs Chinese Herbal Research Article |
| Zdroj: | BioMed Research International, Vol 2015 (2015) BioMed Research International |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2015/102021 |
| Popis: | Huangqin-Tang decoction (HQT) is a classic traditional Chinese herbal formulation that is widely used to ameliorate the symptoms of gastrointestinal disorders, including inflammatory bowel disease (IBD). This study was designed to investigate the therapeutic potential and immunological regulatory activity of HQT in experimental colitis in rats. Using an animal model of colitis by intrarectally administering 2,4,6-trinitrobenzenesulfonic acid (TNBS), we found that administration of HQT significantly inhibited the severity of TNBS-induced colitis in a dose-dependent manner. In addition, treatment with HQT produced better results than that with mesalazine, as shown by improvedweight loss bleeding and diarrhoea scores, colon length, and intestinal inflammation. As for potential immunological regulation of HQT action, the percentages of Th1 and Th17 cells were reduced, but those Th2 and Treg cells were enhanced in LPMCs after HQT treatment. Additionally, HQT lowered the levels of Th1/Th17-associated cytokines but increased production of Th2/Treg-associated cytokines in the colon and MLNs. Furthermore, we observed a remarkable suppression of the Th1/Th17-associated transcription factors T-bet and ROR-γt. However, expression levels of the Th2/Treg-associated transcription factors GATA-3 and Foxp3 were enhanced during treatment with HQT. Our results suggest that HQT has the therapeutic potential to ameliorate TNBS-induced colitis symptoms. This protective effect is possibly mediated by its effects on CD4+T cells subsets. |
| Databáze: | OpenAIRE |
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