D-4F induces heme oxygenase-1 and extracellular superoxide dismutase, decreases endothelial cell sloughing, and improves vascular reactivity in rat model of diabetes
| Autor: | Michael S. Wolin, Saadet Turkseven, Nader G. Abraham, Pawel M. Kaminski, Shuo Quan, Stephen J. Peterson, Frank Zhang, Adam Kruger |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Apolipoprotein B medicine.medical_treatment Intraperitoneal injection Antioxidants Streptozocin Diabetes Mellitus Experimental Superoxide dismutase Rats Sprague-Dawley chemistry.chemical_compound Superoxides Physiology (medical) Internal medicine Diabetes mellitus medicine Animals Aorta biology Apolipoprotein A-I business.industry Superoxide Superoxide Dismutase Endothelial Cells Streptozotocin medicine.disease Rats Up-Regulation Heme oxygenase Endothelial stem cell Oxidative Stress Endocrinology chemistry Vasoconstriction biology.protein Cardiology and Cardiovascular Medicine business Peptides Heme Oxygenase-1 medicine.drug |
| Zdroj: | Circulation. 111(23) |
| ISSN: | 1524-4539 |
| Popis: | Background— Apolipoprotein A1 mimetic peptide, synthesized from D-amino acid (D-4F), enhances the ability of HDL to protect LDL against oxidation in atherosclerotic animals. Methods and Results— We investigated the mechanisms by which D-4F provides antioxidant effects in a diabetic model. Sprague-Dawley rats developed diabetes with administration of streptozotocin (STZ). We examined the effects of daily D-4F (100 μg/100 g of body weight, intraperitoneal injection) on superoxide (O 2 − ), extracellular superoxide dismutase (EC-SOD), vascular heme oxygenase (HO-1 and HO-2) levels, and circulating endothelial cells in diabetic rats. In response to D-4F, both the quantity and activity of HO-1 were increased. O 2 − levels were elevated in diabetic rats (74.8±8×10 3 cpm/10 mg protein) compared with controls (38.1±8×10 3 cpm/10 mg protein; P 2 − levels to 13.23±1×10 3 ( P P Conclusions— We conclude that an increase in circulating endothelial cell sloughing, superoxide anion, and vasoconstriction in diabetic rats can be prevented by administration of D-4F, which is associated with an increase in 2 antioxidant proteins, HO-1 and EC-SOD. |
| Databáze: | OpenAIRE |
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