Cis- and trans-acting elements determining induction of the genes of the gamma-aminobutyrate (GABA) utilization pathway in Saccharomyces cerevisiae
| Autor: | Marcelle Grenson, Bruno André, Driss Talibi |
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| Rok vydání: | 1995 |
| Předmět: |
GABA Plasma Membrane Transport Proteins
Saccharomyces cerevisiae Proteins Transcription Genetic Nitrogen Succinate-Semialdehyde Dehydrogenase (NADP+) Molecular Sequence Data Organic Anion Transporters Saccharomyces cerevisiae Biology Regulatory Sequences Nucleic Acid GATA Transcription Factors Fungal Proteins Transcription (biology) Gene Expression Regulation Fungal Gene expression Genetics Gamma aminobutyrate DNA Fungal Gene Transcription factor gamma-Aminobutyric Acid Regulation of gene expression Reporter gene Base Sequence Membrane Transport Proteins Zinc Fingers Aldehyde Oxidoreductases DNA-Binding Proteins Repressor Proteins Biochemistry 4-Aminobutyrate Transaminase Trans-Activators GATA transcription factor Succinate-Semialdehyde Dehydrogenase Transcription Factors |
| Zdroj: | Nucleic acids research. 23(4) |
| ISSN: | 0305-1048 |
| Popis: | In S. cerevisiae, gamma-aminobutyrate (GABA) induces transcription of the UGA genes required for its utilization as a nitrogen source. Analysis of the 5' region of the UGA1 and UGA4 genes led to the identification of a conserved GC-rich sequence (UASGABA) essential to induction by gamma-aminobutyrate. Alone, this UASGABA element also supported some levels of reporter gene transcription in the presence of gamma-aminobutyrate. To be effective, UASGABA requires two positive-acting proteins that both contain a Cys6-Zn2 type zinc-finger motif, namely pathway-specific Uga3p and pleiotropic Uga35p(Dal81p/DurLp). Further analysis of the UGA4 gene revealed that Gln3p, a global nitrogen regulatory protein containing a GATA zinc-finger domain, is required in order to reach high levels of gamma-aminobutyrate-induced transcription. The Gln3p factor exerts its function mainly through a cluster of 5'-GAT(A/T)A-3'(UASGATA) situated just upstream from UASGABA. The role of Gln3p is less predominant in UGA1 than in UGA4 gene expression. We propose that tight coupling between the UASGABA and UASGATA elements enables the cell to integrate, according to its nitrogen status, the induced expression levels of UGA4. |
| Databáze: | OpenAIRE |
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