Intrasplenic Liver Parenchymal Cells in Conjunction with Low-Dose Rapamycin and Cyclosporine Induce a Unique and Specific Prolongation of Rat Cardiac and Small Bowel Allograft Survival
| Autor: | Vano Baghdassarian, Stanislaw M. Stepkowski, Barry D. Kahan, Lynette J. Dumble, Michael J. Boyle |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Graft Rejection
Male 0301 basic medicine Pathology medicine.medical_specialty Cell Transplantation Liver cytology Lymphocyte medicine.medical_treatment Biomedical Engineering lcsh:Medicine Stimulation Spleen Polyenes Andrology 03 medical and health sciences 0302 clinical medicine Antigen Intestine Small Splenocyte Animals Medicine Sirolimus Heart transplantation Transplantation business.industry Graft Survival lcsh:R Cell Biology Combined Modality Therapy Rats medicine.anatomical_structure 030104 developmental biology Liver Hepatocyte Cyclosporine Heart Transplantation Drug Therapy Combination Lymphocyte Culture Test Mixed business Immunosuppressive Agents 030217 neurology & neurosurgery |
| Zdroj: | Cell Transplantation, Vol 7 (1998) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | These experiments investigated the immunosuppressive properties of liver tissue. Brown Norway (BN; RT1n) rat heart allografts survived in untreated control Wistar Furth (WFu; RTlu) rat recipients for 6.2 ± 1.5 days, while allografts in animals that received rapamycin (RAPA) 0.0075 mg/kg/day and cyclosporine (CsA) 0.375 mg/kg/day delivered for 14 days by continuous intravenous infusion (civi) using osmotic pumps in conjunction with intrasplenic (i.s.) saline survived to 18.4 ± 1.3 days. i.s. addition of 3 M-KCl extracted BN hepatic antigen or unpurified BN hepatocytes (liver parenchymal cells—5 × 107/kg), which exhibited a 4.8% class II antigen expression, and which alone failed to prolong allograft survival (MST = 6.0 ± 1.4 days), increased heart allograft survival to 25.3 ± 2.3 and 27.2 ± 1.9 days, respectively (p < 0.01). Hepatocyte purification using Dynabeads and Percoll reduced class II expression to 0.9% and increased allograft survival to 32.8 ± 1.6 days (p < 0.01). In contrast, the effect of 5 × 108/kg BN erythrocytes, exhibiting only 0.1% class II expression, was much less (23.8 ± 1.9 days). Administration i.s. of BN splenocytes or nonparenchymal liver cells, demonstrated by flow cytometry to exhibit a 47.3 or 55.1% expression of class II antigen, respectively, failed to induce any significant increase in allograft survival (18.4 ± 4.6 and 19.4 ± 0.5 days, respectively). Survival of BN rat small bowel allografts was increased in Lewis (LEW; RTl1) rat recipients treated with RAPA, CsA, and unfractionated BN hepatocytes from 10.2 ± 1.9 to 21.2 ± 1.5 days. Pretreatment with i.s. BN hepatocytes, 14 days prior to harvesting, reduced WFu lymphocyte responses to allogeneic stimulation with BN or ACI spleen cells by 75 and 70%, respectively. Addition of 1 × 105 unpurified donor-specific BN or third-party Buffalo (BUF; RTlb) hepatocytes, but not supernatant, to the responder wells of MLCs resulted in a 61 and 40% suppression, respectively, of the WFu lymphocyte response induced by BN allogeneic stimulation. These findings suggest that while class I MHC expression has a significant role to play in exerting the immunosuppressive effects of hepatocytes, other influences more specific to liver may also prevail. |
| Databáze: | OpenAIRE |
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