Optical tracking of nanometer-scale cellular membrane deformation associated with single vesicle release
| Autor: | Hong Yuan Chen, Yan Guan, Nongjian Tao, Yunze Yang, Ashley Hunt, Fenni Zhang, Shaopeng Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Cell Bioengineering 02 engineering and technology 01 natural sciences Exocytosis Article medicine Tumor Cells Cultured Animals Particle Size Instrumentation Fluid Flow and Transfer Processes Process Chemistry and Technology Vesicle Secretory Vesicles 010401 analytical chemistry Cell Membrane Optical Imaging 021001 nanoscience & nanotechnology Secretory Vesicle 0104 chemical sciences Rats Optical tracking medicine.anatomical_structure Membrane Biophysics Nanoparticles Nanometre 0210 nano-technology Membrane deformation |
| Zdroj: | ACS Sens |
| Popis: | Exocytosis involves interactions between secretory vesicles and plasma membrane. Studying the membrane response is thus critical to the understanding of this important cellular process and to differentiate different mediator release patterns. Here we introduce a label-free optical imaging method to detect the vesicle-membrane interaction induced membrane deformation associated with single exocytosis in mast cells. We show that the plasma membrane expands by a few tens of nm accompanying each vesicle release event, but the dynamics of the membrane deformation varies from cell to cell, which reflect different exocytosis processes. Combining the temporal and spatial information allows us to resolve complex vesicle release processes, such as two vesicle release events that occur closely in time and location. Simultaneous following of a vesicle release with fluorescence and membrane deformation tracking further allows us to determine the propagation speed of the vesicle-release induced membrane deformation along the cell surface, which has an average value of 5.2 ± 1.8 µm/s. |
| Databáze: | OpenAIRE |
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