Primary cilia control the maturation of tubular lumen in renal collecting duct epithelium

Autor: Alexandra Chassot, Yubao Wang, Roberto Montesano, Olga Komarynets, Thomas Ernandez, Eric Féraille, Priscilla Soulié, Soushma Sougoumarin
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Pathology
Physiology
030232 urology & nephrology
Kinesins
Ciliopathies
Mice
0302 clinical medicine
Cilia/metabolism
Cell Movement
Proteoglycans/chemistry
Developmental
RNA
Small Interfering
Protein Kinase C
Kidney
Reabsorption
Podocytes
Cilium
Gene Expression Regulation
Developmental
Tumor Suppressor Proteins/antagonists & inhibitors/genetics/metabolism
Drug Combinations
medicine.anatomical_structure
Kidney Tubules
Proteoglycans
Collagen
Collagen/chemistry
Signal Transduction
medicine.medical_specialty
Primary Cell Culture
Lumen (anatomy)
Biology
Cholangiocyte
03 medical and health sciences
medicine
Collecting/cytology/metabolism
Animals
KIF3A
Cilia
Laminin/chemistry
Kidney Tubules
Collecting
ddc:612
Cell Proliferation
Ion Transport
Tumor Suppressor Proteins
Small Interfering/genetics/metabolism
Cell Biology
Epithelium
030104 developmental biology
Gene Expression Regulation
RNA
Laminin
Kinesin/antagonists & inhibitors/genetics/metabolism
Podocytes/cytology/metabolism
Protein Kinase C/antagonists & inhibitors/genetics/metabolism
Zdroj: American Journal of Physiology. Cell Physiology, Vol. 313, No 1 (2017) pp. C94-C107
ISSN: 0363-6143
Popis: The key role of the primary cilium in developmental processes is illustrated by ciliopathies resulting from genetic defects of its components. Ciliopathies include a large variety of dysmorphic syndromes that share in common the presence of multiple kidney cysts. These observations suggest that primary cilia may control morphogenetic processes in the developing kidney. In this study, we assessed the role of primary cilium in branching tubulogenesis and/or lumen development using kidney collecting duct-derived mCCDN21 cells that display spontaneous tubulogenic properties when grown in collagen-Matrigel matrix. Tubulogenesis and branching were not altered when cilium body growth was inhibited by Kif3A or Ift88 silencing. In agreement with the absence of a morphogenetic effect, proliferation and wound-healing assay revealed that neither cell proliferation nor migration were altered by cilium body disruption. The absence of cilium following Kif3A or Ift88 silencing in mCCDN21 cells did not alter the initial stages of tubular lumen generation while lumen maturation and enlargement were delayed. This delay in tubular lumen maturation was not observed after Pkd1 knockdown in mCCDN21 cells. The delayed lumen maturation was explained by neither defective secretion or increased reabsorption of luminal fluid. Our results indicate that primary cilia do not control early morphogenetic processes in renal epithelium. Rather, primary cilia modulate tubular lumen maturation and enlargement resulting from luminal fluid accumulation in tubular structures derived from collecting duct cells.
Databáze: OpenAIRE
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