Expression of Rv2031c-Rv2626c fusion protein in Mycobacterium smegmatis enhances bacillary survival and modulates innate immunity in macrophages
| Autor: | Zhi‑Kai Xu, Hong Jiang, Li‑Mei Wang, Tai‑Lai Luo, Jian Kang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Recombinant Fusion Proteins Mycobacterium smegmatis Gene Expression Mycobacterium Infections Nontuberculous Biology Biochemistry Microbiology Mycobacterium tuberculosis 03 medical and health sciences Mice Antigen Bacterial Proteins Immunity Genetics Macrophage Animals Tuberculosis Molecular Biology Antigens Bacterial Innate immune system Macrophages biology.organism_classification Fusion protein Immunity Innate 030104 developmental biology RAW 264.7 Cells Oncology Molecular Medicine Tumor necrosis factor alpha |
| Zdroj: | Molecular medicine reports. 17(5) |
| ISSN: | 1791-3004 |
| Popis: | Dormancy-associated antigens encoded by the dormancy survival regulon (DosR) genes are required for survival of Mycobacterium tuberculosis (Mtb) in macrophages. However, mechanisms underlying survival of Mtb in macrophages remains to be elucidated. A recombinant Mycobacterium smegmatis strain (rMs) expressing a fusion protein of two dormancy‑associated antigens Rv2031c and Rv2626c from Mtb was constructed in the present study. In an in vitro culture, growth rate of rMs was lower compared with Ms. A total of 24 h following infection of murine macrophages with rMs or Ms, percentage of viable cells decreased and the number of bacteria in viable cells increased compared with Ms, demonstrating that virulence and intracellular survival of rMs were enhanced. Compared with macrophages infected with Ms, necrosis of macrophages infected with rMs was increased, while apoptosis was inhibited. Macrophages infected with rMs secreted more interferon‑γ and interleukin‑6, but fewer nitric oxide and tumor necrosis factor‑α, compared with macrophages infected with Ms. The present study demonstrated that the fusion protein composed of dormancy‑associated antigens Rv2031c and Rv2626c in Ms serves a physiological function of a dormancy‑associated antigen and modulates innate immunity of host macrophages, therefore favoring intracellular bacillary survival. |
| Databáze: | OpenAIRE |
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