MiR‐3175 promotes epithelial‐mesenchymal transition by targeting Smad7 in human conjunctiva and pterygium
| Autor: | Yihua Wu, Huina Zhang, Zhong Xincao, Han Wu, Juan Ye, Dajing Xia, Hongyi Li, Xin Shi, Jinlong Tang |
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| Rok vydání: | 2020 |
| Předmět: |
Epithelial-Mesenchymal Transition
Conjunctiva Biophysics Biology Pterygium Biochemistry Cell Line Smad7 Protein Pathogenesis 03 medical and health sciences Downregulation and upregulation Cell Movement Structural Biology microRNA Genetics medicine Humans Vimentin Epithelial–mesenchymal transition Molecular Biology Cell Proliferation 030304 developmental biology 0303 health sciences Pterygium (conjunctiva) Transition (genetics) 030302 biochemistry & molecular biology Epithelial Cells Cell Biology medicine.disease eye diseases MicroRNAs medicine.anatomical_structure Cell culture Cancer research sense organs |
| Zdroj: | FEBS Letters. 594:1207-1217 |
| ISSN: | 1873-3468 0014-5793 |
| Popis: | MicroRNAs (miRNAs) are involved in the post-transcriptional regulation of mRNAs. However, the function of miRNAs is unclear in epithelial-mesenchymal transition (EMT) of pterygium, a disease characterized by abnormal fibrovascular proliferation and invasion on the ocular surface. Since MiR-3175 is upregulated in pterygium tissues compared to normal conjunctival tissues, in this study, we investigated the role of MiR-3175 in EMT of a cultured human conjunctival epithelium cell line (CCL) and further validated the findings in pterygium tissues from patients. Our study demonstrates that MiR-3175 promotes proliferation, migration, invasion, and EMT of CCL cells by directly inhibiting Smad7. In conclusion, our data suggested that the MiR-3175-Smad7 axis may play a critical role in pathogenesis and development of pterygium. |
| Databáze: | OpenAIRE |
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