The Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner
| Autor: | Barbara M. Bowen, Kathryn J. Boor, Martin Wiedmann, Celine Nadon, Ute Schwab |
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| Rok vydání: | 2005 |
| Předmět: |
Fluorescent Antibody Technique
Sigma Factor GUS reporter system Biology medicine.disease_cause Microbiology Bacterial Proteins Listeria monocytogenes Genes Reporter Transcription (biology) Genetics medicine RNA Messenger Promoter Regions Genetic Molecular Biology Gene Glucuronidase Regulation of gene expression Messenger RNA fungi RNA Promoter Gene Expression Regulation Bacterial Molecular biology Artificial Gene Fusion RNA Bacterial Trans-Activators Gene Deletion Peptide Termination Factors |
| Zdroj: | FEMS Microbiology Letters. 245:329-336 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1016/j.femsle.2005.03.025 |
| Popis: | Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters (prfAP1, prfAP2, and PplcA). The prfAP2 promoter was previously proposed to be sigma B (sigma(B))-dependent. This hypothesis was tested by creating prfA promoter-gus transcriptional fusions in both L. monocytogenes wild-type (wt) and DeltasigB backgrounds and then measuring (i) beta-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the DeltasigB strain, confirming both growth phase- and sigma(B)-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by sigma(B). |
| Databáze: | OpenAIRE |
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