GC-MS metabolomics analysis of mesenchymal stem cells treated with copper oxide nanoparticles

Autor:	Luisa Mancuso, Cristina Manis, Pierluigi Caboni, Giacomo Cao, Antonio Murgia
Rok vydání:	2016
Předmět:	Adult Male 0301 basic medicine Glyceric acid Surface Properties Health Toxicology and Mutagenesis Metabolite Cell Culture Techniques 010501 environmental sciences Toxicology 01 natural sciences Gas Chromatography-Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Metabolomics Microscopy Electron Transmission Toxicity Tests Humans Particle Size Cytotoxicity Cells Cultured 0105 earth and related environmental sciences chemistry.chemical_classification Reactive oxygen species Mesenchymal Stem Cells Middle Aged Glutamine 030104 developmental biology chemistry Biochemistry Succinic acid Multivariate Analysis Toxicity Metabolome Nanoparticles Female Copper Nuclear chemistry
Zdroj:	Toxicology Mechanisms and Methods. 26:611-619
ISSN:	1537-6524 1537-6516
Popis:	Human exposure to copper oxide (CuO) nanoparticles (NPs) is rapidly increasing and for this reason reliable toxicity test systems are urgently needed. Recently, the acute cytotoxicity of CuO NPs using the new toxicity test based on human bone marrow mesenchymal stem cells (hBMMSCs) has been evaluated. It was shown that CuO NPs are much more toxic when compared to CuO microparticles (MPs). Several studies associate CuO toxicity to a possible alteration of reactive oxygen species (ROS) system. Unluckily, the mechanism that causes the toxicity is still not clear. In this work, the polar metabolite pool of treated cells, at the corresponding IC50 value, for CuO micro and NPs has been studied by gas chromatography coupled to mass spectrometry (GC-MS) and multivariate statistical data analysis. By the same means, differences due to different treatments, on samples, were investigated. Results of discriminant analysis were considered with the aim of finding the relevant metabolites unique for each class. Serine, glyceric acid, and succinic acid were upregulated on samples treated with CuO microparticles, while glutamine was the only discriminant metabolite for the class of samples treated with nanoparticles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bfb1095a7ad23c7855b58e43264fa60 https://doi.org/10.1080/15376516.2016.1220654 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.