Presence of the gypsy (MDG4) retrotransposon in extracellular virus-like particles
| Autor: | Andrej B. Semakin, Boris V. Syomin, Vladimir Tsuprun, Konstantin V. Kandror, Alexander S. Stepanov |
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| Rok vydání: | 1993 |
| Předmět: |
Transcription
Genetic viruses Biophysics Genome Viral complex mixtures Biochemistry Virus-like particle Structural Biology Transcription (biology) Genetics Extracellular Melanogaster Retrotransposon Animals RNA Messenger Molecular Biology Cells Cultured biology virus diseases RNA gypsy Cell Biology RNA Probes biology.organism_classification Blotting Northern Molecular biology Drosophila virilis DNA-Binding Proteins Molecular Weight Microscopy Electron Drosophila melanogaster Retroviridae Nucleic acid DNA Transposable Elements RNA Viral Drosophila DNA Probes Peptides Poly A Plasmids |
| Zdroj: | FEBS letters. 323(3) |
| ISSN: | 0014-5793 |
| Popis: | As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-DNA complexes of gypsy were observed in both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in the obtained VLP preparations. These data accompanied by morphological characteristics of samples testify the presence of intact gypsy VLP in cultured media both from D. melanogaster and D. virilis cultivated cells. |
| Databáze: | OpenAIRE |
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