Structure and adsorption properties of commercial calcium phosphate adjuvant
| Autor: | Cliff T. Johnston, Gnanasiri S. Premachandra, Dongping Jiang, Stanley L. Hem |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Calcium Phosphates
Differential Thermal Analysis medicine.medical_treatment chemistry.chemical_element Calcium Electrochemistry Adsorption Adjuvants Immunologic X-Ray Diffraction Spectroscopy Fourier Transform Infrared medicine Surface charge Point of zero charge Fourier transform infrared spectroscopy Molecular Structure General Veterinary General Immunology and Microbiology Hydroxyl Radical Ligand Public Health Environmental and Occupational Health Durapatite Infectious Diseases chemistry Biochemistry Molecular Medicine Adjuvant Nuclear chemistry |
| Zdroj: | Vaccine. 23:693-698 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2004.06.029 |
| Popis: | Calcium phosphate adjuvant is a commercially available vaccine adjuvant that potentiates the immune response to antigens. Although its name suggests that it is Ca 3 (PO 4 ) 2 , X-ray diffraction, FTIR spectroscopy, thermal analysis and the Ca/P molar ratio identify commercial calcium phosphate adjuvant as non-stoichiometric hydroxyapatite, Ca 10− x (HPO 4 ) x (PO 4 ) 6− x (OH) 2− x , where x varies from 0 to 2. The surface charge is pH-dependent (point of zero charge = 5.5). Consequently, commercial calcium phosphate adjuvant exhibits a negative surface charge at physiological pH and electrostatically adsorbs positively charged antigens. The presence of hydroxyls allows calcium phosphate adjuvant to adsorb phosphorylated antigens by ligand exchange with surface hydroxyls. |
| Databáze: | OpenAIRE |
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