Cloning and sequencing of a rice (Oryza sativa L.) RAPB cDNA using yeast one-hybrid system

Autor:	Mengmin Hong, Zongyang Wang, Jingliu Zhang, Quanhong Yao, Rihe Peng, Yan-Yan Xing
Rok vydání:	1998
Předmět:	Genetics chemistry.chemical_classification Protein subunit CAAT box Biology General Biochemistry Genetics and Molecular Biology Yeast Amino acid Complementation chemistry Complementary DNA General Agricultural and Biological Sciences Gene Peptide sequence General Environmental Science
Zdroj:	Science in China. Series C, Life sciences. 42(4)
ISSN:	1006-9305
Popis:	Cis-acting elements containing CCAAT core sequence are located in 5′ upstream regions of numerous eukaryotic genes. CCAAT-binding factors interact with thesecis acting elements as heteromeric complex and therefore control the gene transcription. CCAAT binding factors contain at least three subunits and each subunit alone cannot bind to CCAAT box. The cloning of a rice cDNA called RAPB which homologizes to yeast HAP2 (one of the subunits in CCAAT-binding factors) using yeast one-hybrid system and functional complementation approaches is reported. The analytic results indicate that the deduced amino acid sequence in the C terminal of RAPB also contains the functional domain of 60 amino acids highly homologous with yeast HAP2, whereas the deduced amino acids in N terminal region differs significantly, and no Gln-rich region is found in the RAPB protein as in HAP2. The Southern blotting analysis demonstrates that only one copy of RAPB gene exists in rice genome.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bf3c3758471fbe68cb8609ac1b80b07 https://pubmed.ncbi.nlm.nih.gov/18763125 Zobrazit plný text záznamu Full text from SpringerLink