Expression of Somatostatin Receptor mRNA in Human Meningiomas and their Implication in in vitro Antiproliferative Activity
| Autor: | Tullio Florio, Renato Spaziante, Alessandra Dorcaratto, Gennaro Schettini, Sara Arena, Paolo Pirani, Gabriella Lapertosa, Valentina Villa, Federica Barbieri, Jean-Louis Ravetti, Stefano Thellung, Alessandro Corsaro, Patrizia Dadati |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Gene Expression Biology Meningioma Gene expression Meningeal Neoplasms Tumor Cells Cultured medicine Humans Protein Isoforms Somatostatin receptor 2 RNA Messenger Receptors Somatostatin Receptor Aged Aged 80 and over Reverse Transcriptase Polymerase Chain Reaction Somatostatin receptor Middle Aged medicine.disease Immunohistochemistry Ki-67 Antigen Real-time polymerase chain reaction Somatostatin Proto-Oncogene Proteins c-bcl-2 Neurology Oncology Cancer research Female Neurology (clinical) Tumor Suppressor Protein p53 Cell Division |
| Zdroj: | Journal of Neuro-Oncology. 66:155-166 |
| ISSN: | 0167-594X |
| DOI: | 10.1023/b:neon.0000013498.19981.55 |
| Popis: | Somatostatin receptors (SSTRs) have been detected in many normal and malignant tissues. This wide expression has been used for diagnostic, prognostic and therapeutic purposes. Five SSTR subtypes (SSTR 1-5) have been identified whose activation is responsible for the signal transduction through many different intracellular pathways. In the present study the expression of SSTR mRNA was determined by reverse-transcriptase (RT)-PCR in 42 meningiomas. About 88% of the tumors analyzed (37/42) were positive for at least one of the five SSTR subtypes displaying a variable pattern of expression of the different SSTR subtypes. SSTRI and SSTR2 were the most frequently mRNA detected (69% and 79% of the sample analyzed, respectively). The other subtypes were found in the 43%, 33% and 33% of cases for SSTR3, SSTR4 and SSTR5, respectively. In 22, out of 42 patients (52%) three or more SSTRs were detected. The expression of the different SSTR subtypes did not correlate with the expression of bcl-2 (apoptosis-associated protein) and MIB-1 (a proliferation marker), assessed by immunohistochemistry in a series of 34 tumor samples, while a correlation between the expression of SSTR3 and p53 was observed (p = 0.08). To evaluate a possible role of SSTR in the control of human meningioma cell proliferation, seven primary cell cultures obtained from fresh meningioma surgical tissues, were analyzed for their proliferative behavior by MTT assay and for their response to SST by [3H]-thymidine incorporation. In four out of six tumors (in one case no SSTR were detected) the treatment with SST caused a significant inhibition of DNA synthesis induced by the tumor-promoter phorbol myristate acetate. The evidence of the expression of SSTRs, mainly of SSTR2, in this series of specimens we analyzed altogether with in vitro antiproliferative effects of SST may open interesting perspectives for the diagnosis and the therapy of meningiomas. |
| Databáze: | OpenAIRE |
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