An Integrated Analysis of Cashmere Fineness lncRNAs in Cashmere Goats
Autor: | Dan Guo, Hui L. Xue, Ze Y Wang, Yuan Y. Zheng, Bo J. Li, Tai Y. Hui, Sheng D. Sheng, Jia M. Sun, Wen L. Bai, Chang Yue, Su L. Guo |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
lcsh:QH426-470 Intermediate filament cytoskeleton RNA-Seq Computational biology Biology Genome Article differently expressed genes 03 medical and health sciences 0302 clinical medicine lncRNA Gene expression Genetics Animals Gene Regulatory Networks lncRNA–targets RNA Messenger KEGG Gene Genetics (clinical) Skin Messenger RNA Goats cashmere fineness RNA Computational Biology High-Throughput Nucleotide Sequencing Lipid Metabolism lcsh:Genetics MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis RNA Long Noncoding RNA-seq Hair Follicle |
Zdroj: | Genes Genes, Vol 10, Iss 4, p 266 (2019) Volume 10 Issue 4 |
ISSN: | 2073-4425 |
Popis: | Animal growth and development are regulated by long non-coding RNAs (lncRNAs). However, the functions of lncRNAs in regulating cashmere fineness are poorly understood. To identify the key lncRNAs that are related to cashmere fineness in skin, we have collected skin samples of Liaoning cashmere goats (LCG) and Inner Mongolia cashmere goats (MCG) in the anagen phase, and have performed RNA sequencing (RNA-seq) approach on these samples. The high-throughput sequencing and bioinformatics analyses identified 437 novel lncRNAs, including 93 differentially expressed lncRNAs. We also identified 3,084 differentially expressed messenger RNAs (mRNAs) out of 27,947 mRNAs. Gene ontology (GO) analyses of lncRNAs and target genes in cis show a predominant enrichment of targets that are related to intermediate filament and intermediate filament cytoskeleton. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, sphingolipid metabolism is a significant pathway for lncRNA targets. In addition, this is the first report to reveal the possible lncRNA&ndash mRNA regulatory network for cashmere fineness in cashmere goats. We also found that lncRNA XLOC_008679 and its target gene, KRT35, may be related to cashmere fineness in the anagen phase. The characterization and expression analyses of lncRNAs will facilitate future studies on the potential value of fiber development in LCG. |
Databáze: | OpenAIRE |
Externí odkaz: |