Blood speaks: Personalised medicine profiling for heart failure patients

Autor:	Mark A. Sussman, Fareheh Firouzi
Rok vydání:	2020
Předmět:	WT wild type Research paper CSC cardiac stem cell EPOR Erythropoietin receptor LAD Left anterior descending coronary artery RIVA Ang-1 Angiopoeitin 1 Clonal hematopoiesis of indeterminate pathology Angiogenesis induction MNC Mononuclear cells SH2B3 LNK [Src homology 2-B3 (SH2B3)] belongs to a family of SH2-containing proteins with important adaptor functions Coronary bypass surgery Profiling (information science) CABG CHIP Clonal hematopoiesis of indeterminate potential PI3K Phosphoinositide-3-Kinase m mouse BM Bone marrow PPMC Pearson Product Moment Correlation CHIP BrdU Brome deoxyuridine NR non-responder IGF-1 Insuline-like Growth Factor 1 AUC Area under curve BMMNC Bone marrow mononuclear cell General Medicine KSL mouse bone marrow stem cell subpopulation c-KIT+ Sca-I+ lin EGF Epidermal growth factor GFP Green fluorescent protein Public Health and Health Services MI myocardial infarction lcsh:Medicine (General) NGS Next Generation Sequencing medicine.medical_specialty 2019-20 coronavirus outbreak Clinical Sciences 6MWT 6-Minute Walk Test InDel mutation insertion or deletion variant General Biochemistry Genetics and Molecular Biology CLARA Clustering for Large Applications PDFR Platelet derived growth factor receptor c-KIT/CD117 Stem Cell Factor Receptor c-KIT CD117 HR Hazard ratio IGFBP Insuline-like growth factor binding proteine IHG Analysis performed in accordance with ISHAGE guidelines RFI Reactome functional interaction Machine learning Humans LAS Longitudinal axis strain Intensive care medicine DE Differential gene expression Heart Failure GWAS Genome wide association study CI Confidence interval hu human R responder lcsh:R Myocardial regeneration FACS Fluorescence activated cell sorter DNAseq desoxyribonucleid acid sequencing SNP Single nucleotide polymorphism variant RT-PCR Reverse transcriptase polymerase chain reaction medicine.disease Cardiac stem cell therapy GMP Good Manufacturing Practice Post myocardial infarction heart failure SDF-1 Stromal Cell-derived Factor 1 FDR False discovery rate PDGF Platelet derived growth factor EPO Erythropoietin MRI Magnetic resonance imaging VEGFR Vascular Endothelial Growth Factor Receptor AUR Arch user repository LCRC Loss of cardiac regeneration capacity GADPH Glyceraldehyde 3 phosphate dehydrogenase lcsh:Medicine LVESD Left Ventricular End Systolic Dimension VCA Virus-Capsid-Antigen CEC Circulating endothelial cells CEC panel CDs measured in PB RNASeq Ribonucleid acid sequencing SUSAR Suspected Unexpected Serious Adverse Reaction GATA4 Transcriptional activator that binds to the consensus sequence 5′-AGATAG-3’ IL Interleukin Precision Medicine CAP-EPC Concentrated Ambient Particles – Endothelial Progenitor Cells lcsh:R5-920 VEGF Vascular Endothelial Growth Factor SH2B3 STEMI ST- segment Elevation Infarction CD Cluster of Differentiation SCF Stem Cell Factor PBMNC Peripheral blood mononuclear cell CABG Coronary Artery Bypass Graft HSC Hemopoeitic stem cell ML Machine learning CFU Colony-forming unit PBMNC mononuclear cells isolated from peripheral blood LNK SH2B adapter protein 3 (lymphocyte adapter protein) Coronavirus disease 2019 (COVID-19) BMSC Bone marrow stem cells Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) LVEDV Left Ventricular End Diastolic Volume RWMS Regional wall motion score qPCR Quantitative polymerase chain reaction ROC Receiver operating characteristics CPC cardiac progenitor cell medicine EGFR Epidermal growth factor receptor ICH GCP Tripartite Guidelines Guideline for Good Clinical Practice TiCoNE Time course network enrichment ELISA Enzyme-Linked Immunosorbent Assay PEI Paul-Ehrlich Institute LVEF Left Ventricular Ejection Fraction business.industry HIF Hypoxia-Inducible Factor transcription factor PB Peripheral blood EPC Endothelial Progenitor Cells EPC panel CDs measured in PB t-SNE t-distributed stochastic neighbour embedding ANCOVA Analysis of covariance WGCNA Weighted gene coexpression network analysis Heart failure TNF Tumor Necrosis Factor PCR Polymerase chain reaction business
Zdroj:	EBioMedicine EBioMedicine, Vol 58, Iss, Pp 102900-(2020)
ISSN:	2352-3964
Popis:	Background Bone marrow stem cell clonal dysfunction by somatic mutation is suspected to affect post-infarction myocardial regeneration after coronary bypass surgery (CABG). Methods Transcriptome and variant expression analysis was studied in the phase 3 PERFECT trial post myocardial infarction CABG and CD133+ bone marrow derived hematopoetic stem cells showing difference in left ventricular ejection fraction (∆LVEF) myocardial regeneration Responders (n=14; ∆LVEF +16% day 180/0) and Non-responders (n=9; ∆LVEF -1.1% day 180/0). Subsequently, the findings have been validated in an independent patient cohort (n=14) as well as in two preclinical mouse models investigating SH2B3/LNK antisense or knockout deficient conditions. Findings 1. Clinical: R differed from NR in a total of 161 genes in differential expression (n=23, q Graphical abstract Image, graphical abstract
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bec689472e48af1a08ab0b6c96a33df https://pubmed.ncbi.nlm.nih.gov/32629392 Zobrazit plný text záznamu