Identification of sustainable trypsin active‐site inhibitors from Nigrospora sphaerica strain AVA‐1
| Autor: | Varun Thachan Kundil, Debarshi Kar Mahapatra, E. Jayadevi Variyar, Aravind Ayyolath, Tomy Muringayil Joseph, Anoop Kallingal, Józef Haponiuk |
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| Rok vydání: | 2021 |
| Předmět: |
Loranthaceae
Applied Microbiology and Biotechnology Antioxidants Nigrospora sphaerica Plant use of endophytic fungi in defense chemistry.chemical_compound Ascomycota Endophytes medicine Trypsin chemistry.chemical_classification biology Dendrophthoe falcata General Medicine biology.organism_classification Bioactive compound Enzyme assay Molecular Docking Simulation medicine.drug_formulation_ingredient Enzyme chemistry Biochemistry Fermentation biology.protein Trypsin Inhibitors Quercetin medicine.drug |
| Zdroj: | Journal of Basic Microbiology. 61:709-720 |
| ISSN: | 1521-4028 0233-111X |
| DOI: | 10.1002/jobm.202100221 |
| Popis: | Trypsin is a protein-digesting enzyme that is essential for the growth and regeneration of bone, muscle, cartilage, skin, and blood. The trypsin inhibitors have various role in diseases such as inflammation, Alzheimer's disease, pancreatitis, rheumatoid arthritis, cancer prognosis, metastasis and so forth. From 10 endophytic fungi isolated, we were able to screen only one strain with the required activity. The fungus with activity was obtained as an endophyte from Dendrophthoe falcata and was later identified as Nigrospora sphaerica. The activity was checked by enzyme assays using trypsin. The fungus was fermented and the metabolites were extracted and further purified by bioassay-guided chromatographic methods and the compound isolated was identified using gas chromatography-mass spectrometry. The compound was identified as quercetin. Docking studies were employed to study the interaction. The absorption, distribution, metabolism, and excretion analysis showed satisfactory results and the compound has no AMES and hepatotoxicity. This study reveals the ability of N. sphaerica to produce bioactive compound quercetin has been identified as a potential candidate for trypsin inhibition. The present communication describes the first report claiming that N. sphaerica strain AVA-1 can produce quercetin and it can be considered as a sustainable source of trypsin active-site inhibitors. |
| Databáze: | OpenAIRE |
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