Small intestine remodeling in male Goto–Kakizaki rats

Autor: Fabio Takeo Sato, Gilson Masahiro Murata, Aline Rosa Marosti, Carla Roberta de Oliveira Carvalho, Rui Curi, Joice Naiara Bertaglia Pereira
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Blood Glucose
Male
medicine.medical_specialty
Physiology
Duodenum
Crypt
small intestine morphology
Myenteric Plexus
Ileum
030204 cardiovascular system & hematology
digestive system
lcsh:Physiology
Muscle hypertrophy
RESISTÊNCIA À INSULINA
Jejunum
03 medical and health sciences
0302 clinical medicine
Insulin resistance
enteric nervous system
Physiology (medical)
Internal medicine
Intestine
Small
medicine
Animals
Rats
Wistar
Gastrointestinal Transit
GK rats
lcsh:QP1-981
Chemistry
digestive
oral
and skin physiology
Original Articles
Submucous Plexus
medicine.disease
Small intestine
Disease Models
Animal
Endocrinology
medicine.anatomical_structure
Diabetes Mellitus
Type 2
inflammation
Cytokines
Enteric nervous system
Original Article
type 2 diabetes
Inflammation Mediators
Insulin Resistance
030217 neurology & neurosurgery
Zdroj: Physiological Reports, Vol 9, Iss 3, Pp n/a-n/a (2021)
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Physiological Reports
Popis: Background Obesity is associated with the development of insulin resistance (IR) and type‐2 diabetes mellitus (T2DM); however, not all patients with T2DM are obese. The Goto–Kakizaki (GK) rat is an experimental model of spontaneous and non‐obese T2DM. There is evidence that the intestine contributes to IR development in GK animals. This information prompted us to investigate small intestine remodeling in this animal model. Methods Four‐month‐old male Wistar (control) and GK rats were utilized for the present study. After removing the small intestine, the duodenum, proximal jejunum, and distal ileum were separated. We then measured villi and muscular and mucosa layer histomorphometry, goblet cells abundance, total myenteric and submucosal neuron populations, and inflammatory marker expression in the small intestinal segments and intestinal transit of both groups of animals. Key Results We found that the GK rats exhibited decreased intestinal area (p
Goto–Kakizaki (GK) rat is an experimental model of spontaneous and non‐obese type‐2 diabetes mellitus—T2DM).GK rats exhibit decreased small intestinal area, increased crypt depth, villi height and thickness, and muscular layer thickness, increased content of IL‐1β and NF‐κB p65, decreased density of submucosal neurons, myenteric and submucosal neuronal and ganglionic hypertrophy, and decreased intestinal transit.The development of IR and T2DM in GK rats is associated with small intestine remodeling with marked changes in morphology, enteric nervous system, and transit.
Databáze: OpenAIRE
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