Choline in Infant Formula and Adult Nutritionals by Ion Chromatography and Suppressed Conductivity: First Action 2012.20
| Autor: | Lillian Chen, Kassandra Oates, Deepali Mohindra, Jeffrey S. Rohrer, Brian M. De Borba, Dawn Dowell |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Ion chromatography Conductivity Chemistry Techniques Analytical Choline Analytical Chemistry chemistry.chemical_compound Hydrolysis Cations Animals Humans Environmental Chemistry Microwaves Food Formulated Pharmacology Chromatography Las vegas Infant Reproducibility of Results Water Repeatability Reference Standards Chromatography Ion Exchange Infant Formula Milk Infant formula chemistry Calibration Acid hydrolysis Soybeans Agronomy and Crop Science Food Science |
| Zdroj: | Journal of AOAC INTERNATIONAL. 96:1400-1406 |
| ISSN: | 1944-7922 1060-3271 |
| DOI: | 10.5740/jaoacint.13-177 |
| Popis: | Single-laboratory validation (SLV) data from a method for the determination of choline in infant formula and adult nutritionals by ion chromatography (IC) and suppressed conductivity were generated and presented to the Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) Expert Review Panel (ERP) at the AOAC Annual Meeting held in Las Vegas, NV, during September 30 to October 3, 2012. The ERP reviewed the data and concluded that the data met the standard method performance requirements (SMPRs) established and approved the method as AOAC Official First Action. At the ERP's request, a second, full SLV was performed on 17 SPIFAN matrixes that included fortified and placebo products. Prior to IC analysis, microwave-assisted acid hydrolysis was used to digest and release bound choline from powder and ready-to-feed (RTF) infant formula and adult nutritional samples. Following hydrolysis, separation of choline from common cations was achieved on a Thermo ScientificTM DionexTM IonPacTM CS19 column followed by suppressed conductivity detection. Total choline was measured and reported as the choline ion in mg/100 g reconstituted material or RTF as-is. The system was calibrated over the analytical range specified in the SMPR (2–250 mg/100 g). Recoveries of spiked samples at 50 and 100% of the fortified choline amounts ranged from 93.1 to 100.7% with RSDs ≤6.7% for product containing |
| Databáze: | OpenAIRE |
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