Demonstration of p53 protein and TP53 gene mutations in oligodendrogliomas
| ISSN: | 0959-8049 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9be6ce7653c3ac09ee327620e94520a1 https://pubmed.ncbi.nlm.nih.gov/9038605 |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....9be6ce7653c3ac09ee327620e94520a1 |
| Autor: | Christian Hagel, R. Jung, Karin Milde-Langosch, Dimitrios Stavrou, George Laking, S. Scheil, Rudolf Laas |
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Cancer Research Tumor suppressor gene Oligodendroglioma Biology Gene mutation medicine.disease_cause Polymerase Chain Reaction Immunoenzyme Techniques Exon medicine Humans neoplasms Gene TP53 Gene Mutation Polymorphism Single-Stranded Conformational Brain Neoplasms Single-strand conformation polymorphism Middle Aged medicine.disease Genes p53 Molecular biology Neoplasm Proteins Oncology Mutation Cancer research Female Tumor Suppressor Protein p53 Carcinogenesis |
| Zdroj: | European journal of cancer (Oxford, England : 1990). (13) |
| ISSN: | 0959-8049 |
| Popis: | Paraffin embedded tissue of 84 oligodendrogliomas (63 primary tumours, 21 recurrences), 21 glioblastomas with oligodendroglial growth pattern (15 primaries, 6 recurrences) and 17 mixed gliomas was investigated for the presence of mutations in exons 5-9 by means of single stranded conformation polymorphism (SCCP), temperature gradient gel electrophoresis (TGGE) and direct DNA sequencing. In parallel, p53 protein accumulation was determined by means of immunohistochemistry. The percentage of mutations was found to be higher than previously reported (6 of 44 grade II oligodendrogliomas, 4 of 19 grade III oligodendrogliomas, 4 of 15 glioblastomas). In 4 cases, the mutations lead to distinct changes in the primary or secondary structure of the protein (cysteine-->tyrosine, proline-->leucine) and were associated with marked accumulation of p53 protein. A significant correlation between p53 protein accumulation and TP53 gene aberrations was found (P < 0.001), although p53 protein accumulation was detected more often than TP53 gene anomalies, indicating that factors other than TP53 gene mutation may also lead to a p53 protein accumulation in the tumour cells. A significant correlation was found for p53 protein accumulation and tumour grade but not TP53 gene mutations. In conclusion, evaluation of p53 protein accumulation reflected the clinical course of oligodendrogliomas better than the mere presence of TP53 gene mutations. |
| Databáze: | OpenAIRE |
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