Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems
| Autor: | Le Wang, Wu Bo, Xiu-Lan Xu, Xingchun Zhao, Li Jiang, Yi-Cheng Liu, An-Quan Ji, Guang-Feng Zhang, Chen Man, Jian Ye |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Forensic Genetics Genotype Computational biology Biology DNA sequencing Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Genetics Humans 030216 legal & forensic medicine Genotyping Alleles Massive parallel sequencing High-Throughput Nucleotide Sequencing Reproducibility of Results Sequence Analysis DNA DNA Fingerprinting Forensic science genomic DNA 030104 developmental biology DNA profiling Microsatellite Microsatellite Repeats |
| Zdroj: | Journal of forensic sciences. 63(6) |
| ISSN: | 1556-4029 |
| Popis: | Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef™ and S5™ XL systems using an early access 25-plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA. In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef™ and the Ion S5™ XL systems provided an alternative and promising approach for forensic STR genotyping. |
| Databáze: | OpenAIRE |
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